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REVIEW article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1554965

Applying nanopore sequencing in the etiological diagnosis of bloodstream infection

Provisionally accepted
Yiqun Liao Yiqun Liao 1JunJie Gong JunJie Gong 1Xiaoling Wang Xiaoling Wang 1*Puwen Chen Puwen Chen 2*Qinxing Chi Qinxing Chi 2*Xiaohong Chen Xiaohong Chen 1*
  • 1 First Affiliated Hospital of Gannan Medical University, Ganzhou, China
  • 2 The First School of Clinical Medicine, Gannan Medical University, Ganzhou, China, Ganzhou, China

The final, formatted version of the article will be published soon.

    Bloodstream infection (BSI) is a systemic infectious disease that can lead to shock, disseminated intravascular coagulation, multiorgan failure, and even death.Blood culture is considered the gold standard for the etiological diagnosis of BSI; however, blood culture is time-consuming and has a low positivity rate, which has limited its utility for early and rapid clinical diagnosis. Nanopore sequencing technology (NST), a third-generation sequencing method, offers rapid detection, real-time single-molecule sequencing, and ultra-long reads. These features enable the prompt detection of pathogens and the analysis of drug-resistant genes and genomic characteristics, thereby optimizing the clinical diagnosis and treatment of BSI. In this article, we summarize the application of NST in the etiological diagnosis of BSI.

    Keywords: Bloodstream infection, Etiological diagnosis, high-throughput sequencing, nanopore sequencing, whole genome sequencing

    Received: 03 Jan 2025; Accepted: 03 Feb 2025.

    Copyright: © 2025 Liao, Gong, Wang, Chen, Chi and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xiaoling Wang, First Affiliated Hospital of Gannan Medical University, Ganzhou, China
    Puwen Chen, The First School of Clinical Medicine, Gannan Medical University, Ganzhou, China, Ganzhou, China
    Qinxing Chi, The First School of Clinical Medicine, Gannan Medical University, Ganzhou, China, Ganzhou, China
    Xiaohong Chen, First Affiliated Hospital of Gannan Medical University, Ganzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.