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ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Microorganisms in Vertebrate Digestive Systems

Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1550971

Investigating the Renoprotective Effects of Polygonatum sibiricum Polysaccharides (PSP) on D-Galactose-Induced Aging Mice: Insights from Gut Microbiota and Metabolomics Analyses

Provisionally accepted
Rui Huang Rui Huang 1Runli Che Runli Che 1Taoli Sun Taoli Sun 1Wei Xie Wei Xie 2*Shuihan Zhang Shuihan Zhang 1*
  • 1 Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Anhui Province, China
  • 2 The First People's Hospital of Changde City, Changde, Hunan Province, China

The final, formatted version of the article will be published soon.

    This study investigated the renoprotective mechanisms of Polygonatum sibiricum polysaccharides (PSP) in D-galactose (D-gal)-induced aging mice through gut microbiota and metabolomics analysis. The mice were divided into five groups (control, model, vitamin C, low-dose PSP, and high-dose PSP) and treated for 8 weeks. Through histopathological analysis (H&E and Masson's trichrome staining), 16S rRNA sequencing, and non-targeted metabolomics approaches, the study demonstrated that PSP treatment effectively ameliorated renal pathological alterations, particularly reducing tubular atrophy, epithelial cell swelling, and interstitial collagen fiber deposition. PSP modulated gut microbiota by increasing beneficial bacteria (Lactobacillus and Bifidobacterium) and altered 23 metabolites associated with five key metabolic pathways, including amino acid metabolism and sphingolipid metabolism.The integrative analysis demonstrated strong correlations between gut bacteria abundances and metabolite levels, suggesting that PSP protects against age-related kidney damage by regulating the gut-kidney axis through modifications in both gut microbiota composition and metabolic pathways.

    Keywords: Polygonatum sibiricum polysaccharides, Renoprotection, Aging, Gut Microbiota, Metabolomics

    Received: 03 Jan 2025; Accepted: 21 Mar 2025.

    Copyright: © 2025 Huang, Che, Sun, Xie and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wei Xie, The First People's Hospital of Changde City, Changde, Hunan Province, China
    Shuihan Zhang, Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Anhui Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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