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REVIEW article
Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1550601
This article is part of the Research Topic Women in Infectious Agents and Disease: 2024 View all 6 articles
Diagnosis of bovine rotavirus: an overview of currently available methods
Provisionally accepted- 1 Xining Animal Disease Control Center, Xining, China
- 2 Qinghai University, Xining, Qinghai Province, China
Bovine rotavirus (BRV) is a significant pathogen that causes diarrhea in calves, profoundly impacting the cattle industry and resulting in substantial economic losses. Currently, the established diagnostic approaches for BRV primarily include etiological methods, such as electron microscopy, virus isolation, and culture; serological methods, including enzyme-linked immunosorbent assay (ELISA), latex agglutination test (LAT), and immunofluorescence techniques; and molecular biological methods, such as reverse transcription polymerase chain reaction (RT-PCR), real-time quantitative PCR (qPCR), and loop-mediated isothermal amplification (LAMP), as well as next-generation sequencing (NGS) technology. This review summarizes the current diagnostic methods for BRV, discusses their advantages and disadvantages, and presents future perspectives on BRV diagnosis, aiming to provide valuable references for the effective diagnosis and control of BRV-related diseases.
Keywords: Bovine rotavirus, diagnosis, Etiological methods, Serological methods, Molecular biological methods
Received: 23 Dec 2024; Accepted: 17 Feb 2025.
Copyright: © 2025 Meng, Huang, Li, FU, Wang and Kang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ru Meng, Xining Animal Disease Control Center, Xining, China
Shu Qin Wang, Xining Animal Disease Control Center, Xining, China
Ming Kang, Qinghai University, Xining, 810016, Qinghai Province, China
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