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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1548106
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Multidrug-resistant Pseudomonas aeruginosa (MDR-PA) wound infections pose a significant global public health threat. The prevalence of antibiotic resistance, such as cephalosporins, carbapenems, and short-lived antibiotic activity in wounds necessitate innovative therapeutic strategies with potent antibacterial synergy and extended-release formulations. We developed a biocompatible CHX containing nanofilm (CNF) encapsulating chlorhexidine acetate (CHX) integrating antibacterial agents for sustained release based on Tween 80, propylene glycol, isopropyl myristate and CS-PVA liquid film system.. The results revealed a good particle size (59.27 nm) and stable zeta potential (-15.2 mV) that are suitable for wound healing applications. Additionally, the nanofilm was stable, exhibited long-term stability (24 months) and sustained release in simulated wound fluid. The in vitro results showed that this nanofilm does not induce dose-related toxic effects and displays a better antibacterial effect that occurs more quickly, 2 times greater than that of CHX in vitro. This safe nanofilm enhances antibacterial activity against P. aeruginosa for 14 days, modulates the immune response, and accelerates skin wound healing in vivo. These insights into multifunctional nanofilm designs for improved antibacterial effects and sustained release suggest promising clinical applications.
Keywords: Multidrug-resistant bacterial, Pseudomonas aeruginosa, Nanofilm, Antibacterial effect, Wound heal
Received: 08 Jan 2025; Accepted: 24 Mar 2025.
Copyright: © 2025 Lu, Lu, Wang, Wei, Chen, Hailin, Liu, Ye, Liu, Lin, Liu, Bi, Zou, Zhang, Sun and Tong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongwu Sun, Third Military Medical University, Chongqing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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