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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
Volume 16 - 2025 |
doi: 10.3389/fmicb.2025.1547021
This article is part of the Research Topic Advancements and Challenges in Mpox Research View all articles
Preparation and application evaluation of monoclonal antibodies against Monkeypox virus A29 protein
Provisionally accepted
Xiangjun Ai 1
Xinlan Zhang 1
Ye Xu 2* Chufan Tan 1,2* Chen Duan 1* Nami Liao 1*
Junxi Liu 1 Yilan Qiu 2,3*
Defu Hou 1,2 Qinlin Wang 1,2*
Rushi Liu 1,2*- 1 College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
- 2 Hunan Xuxiang Biotechnology Co., Ltd., Changsha, China
- 3 College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
Monkeypox virus (MPXV) is a DNA virus belonging to the Orthopoxvirus genus of the Poxviridae family. It causes symptoms similar to Smallpox virus and is a zoonotic virus with widespread prevalence. Antigen detection is a fast and effective detection method. The MPXV A29 protein not only plays a important role in the virus lifecycle but also serves as a promising target for developing specific antibodies, which have significant potential for application in the diagnosis of MPXV. The coding sequences of the MPXV A29 protein, Cowpox virus(CPXV) 163 protein homolog and Vaccinia virus (VACV) A27 protein homolog were chemically synthesized, and all three recombinant proteins were expressed in Escherichia coli (BL21 Star). Then, the recombinant A29 protein was used as an antigen to immunize BALB/c mice, and a total of 4 monoclonal antibodies against A29 protein were obtained. Using two homologous proteins as reverse screening systems, a specific monoclonal antibody, mAb-25, against the A29 protein was screened. Then, the mAb-25 was used as a coating antibody to pair with other monoclonal antibodies, leading to the identification of a well-matched antibody pair. A chemiluminescence enzyme immunoassay (CLEIA) and immunochromatographic gold assay were subsequently established using the optimal antibody pair. The experimental results indicate that monoclonal antibodies against the A29 protein hold significant potential for application in the diagnosis of MPXV.
Keywords: Monkeypox Virus (MPXV), monoclonal antibodies, chemiluminescence enzyme immunoassay, Colloidal gold immunochromatography, Antigen detection
Received: 17 Dec 2024; Accepted: 17 Jan 2025.
Copyright: © 2025 Ai, Zhang, Xu, Tan, Duan, Liao, Liu, Qiu, Hou, Wang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ye Xu, Hunan Xuxiang Biotechnology Co., Ltd., Changsha, China
Chufan Tan, College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
Chen Duan, College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
Nami Liao, College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
Yilan Qiu, College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
Qinlin Wang, College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
Rushi Liu, College of Medical Technology and Translational Medicine, Hunan Normal University, Changsha, China
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