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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1546550
This article is part of the Research Topic Expert Opinions: Save the Microbes to Save the Planet View all 12 articles
Histopathological observation and HSP genes expression analysis of Puccinia triticina under high-temperature stress in Jinmai region
Provisionally accepted- College of Plant Protection, Shanxi Agricultural University, Taiyuan, China
Wheat leaf rust is one of the most significant diseases affecting wheat. With the increase of global average temperature, the areas where wheat leaf rust can survive winter and persist through summer have been expanding. And high-temperature resistant isolates were identified within the natural population. In this study, two high-temperature resistant strains and two temperature-sensitive strains were selected from 41 isolates collected from Shanxi Province between 2021 and 2022. The analysis of disease severity differences among the four strains at different temperatures revealed that the differences were most significant at 26℃ (P<0.05). Therefore 26℃ was selected as the optimal temperature for high-temperature stress. Subsequently, histopathological observations were conducted on the four Pt strains, and the expression levels of five genes were determined, including PtHsp90-1, PtHp, PtHspHSS1, PtHsp60 and PtHspSSB. Histopathological observations showed that compared with the temperature-sensitive strains, the number of haustorial mother cells and haustoria of high-temperature resistant was not significantly affected by high-temperature stress (P<0.05).However, the number of hyphal branches of four strains was less affected, indicating that formation of haustorial mother cells and haustoria were the key stages in resistance to heat stress. Further analysis revealed that the expression of five genes reached their maximum at 6 hpi under hightemperature stress. The results indicated that HSP genes play a crucial role in high-temperature resistance during the germination stage of Pt urediospores. However, the specific molecular mechanism in wheat leaf rust required further study and verification. In conclusion, the early germination of urediospores (0-12 h) and the formation of haustorial mother cells and haustoria (12-24 h) were key stages in resisting high-temperature stress in wheat leaf rust.
Keywords: Puccinia triticina, temperature sensitivity, histopathology, Infection process, Heat shock protein
Received: 17 Dec 2024; Accepted: 11 Mar 2025.
Copyright: © 2025 Liu, Zhao, Guan, Liu, Gong and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Minjie Liu, College of Plant Protection, Shanxi Agricultural University, Taiyuan, China
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