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METHODS article
Front. Microbiol.
Sec. Food Microbiology
Volume 16 - 2025 |
doi: 10.3389/fmicb.2025.1545266
Gene manipulation in Oenococcus oeni based on a newly applicable gene gun technology
Provisionally accepted
Chen Mengrong 
Chen Qiling *- Xinjiang Agricultural University, Ürümqi, China
Oenococcus oeni is an important engineering microbe in winemaking. Detailed knowledge of its growth and metabolism in harsh wine environments could contribute to breeding elite O. oeni varieties. However, further studies on this topic do not appear to be sustained due to the lack of stable and reproducible technology to perform gene manipulation on O. oeni. Therefore, this research was designed to study gene function by exploring a newly applicable transformation technique that could perform stably and reproducibly on O. oeni. By using gene gun technology with detonation nanodiamonds as a plasmid DNA carrier, we achieved stable and reproducible plasmid DNA transformation in O. oeni. In addition, the overexpression of the OEOE_RS01070 gene, which showed an overall high single nucleotide polymorphism density by whole-genome alignments of six O. oeni mutants obtained from our previous research, significantly improved the survival ratio of O. oeni SX-1b at pH 2.5.
Keywords: Oenococcus oeni, Gene Gun, detonation nanodiamonds, stable genetic transfe, Wine
Received: 14 Dec 2024; Accepted: 17 Jan 2025.
Copyright: © 2025 Mengrong and Qiling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Chen Qiling, Xinjiang Agricultural University, Ürümqi, China
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