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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1543210
This article is part of the Research Topic Unravelling Microbial Interactions in Plant Health and Disease Dynamics View all 8 articles
Global characterization of GH3 family glycoside hydrolase genes in Fusarium verticilloides and functional analysis of FvGH3-6
Provisionally accepted- Hebei Agricultural University, Baoding, China
To clarify the roles of glycoside hydrolase 3 (GH3) family genes in the growth, development, and pathogenicity of Fusarium verticillioides, GH3 family genes were identified in the genome by bioinformatics software, and their expression levels in the infection process of F. verticillioides were analyzed using transcriptome data. The FvGH3-6 gene was knocked out and complemented via genetic transformation to explore the role of F. verticillioides. The results demonstrated that a total of 19 GH3 family genes were identified in the genome of F. verticillioides, which were located on 11 chromosomes, encoding amino acids ranging from 559 to 1034, with relative molecular weights between 61.20 and 113.97 kDa, and containing 1 - 6 exons. Transcriptome data indicated that during the infection of maize kernels by F. verticillioides, the expression of nine genes, including FvGH3-6, was upregulated at different stages. Knockout of the FvGH3-6 gene did not impact the mycelial growth rate of F. verticillioides but reduced the sporulation ability. Compared with the wild type, the pathogenicity of FvGH3-6 knockout mutants towards maize grains and stems was weakened. The above results suggest that the glycoside hydrolase gene family participates in the pathogenicity of F. verticillioides, and the FvGH3-6 gene plays a significant role in the conidia production and pathogenicity of F. verticillioides.
Keywords: Fusarium verticillioides, Glycoside hydrolase 3, Expression pattern, Functional Analysis, pathogenicity
Received: 11 Dec 2024; Accepted: 21 Feb 2025.
Copyright: © 2025 Zhang, Duan, Shi, Sun, Liu, Cao and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhiyan Cao, Hebei Agricultural University, Baoding, China
Jingao Dong, Hebei Agricultural University, Baoding, China
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