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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 |
doi: 10.3389/fmicb.2025.1541800
The gastric microbiome altered by A4GNT deficiency in mice
Provisionally accepted
Dawei Gong 1,2
Yuqiang Gao 3* Rui Shi 4* Xiaona Xu 5*
Mengchao Yu 5 Shumin Zhang 1* Lili Wang 5*
Quanjiang Dong 5*- 1 Fourth People’s Hospital of Jinan, Jinan, Shandong Province, China
- 2 Central Laboratories, the affiliated Qingdao Municipal Hospital of Dalian Medical University, Central Laboratories, the affiliated Qingdao Municipal Hospital of Dalian Medical University, China
- 3 Department of Gastroenterology, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
- 4 Qingdao University Medical College, Qingdao, Shandong Province, China
- 5 Central Laboratories, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
Background: Selective antimicrobial effects have been found for α1,4-linked N-acetylglucosamine residues at the terminus of O-glycans attached to a core protein of gastric gland mucin. A4gnt encodes α1,4-N-acetylglucosaminyl transferase, which is responsible for the biosynthesis of α1,4-linked N-acetylglucosamine. The impact of A4GNT on the establishment and homeostasis of the gastric microbiome remains to be clarified. The aim of this study was to characterize the gastric microbiome in mice deficient for the production of α1,4-linked N-acetylglucosamine.The gastric microbiome within A4gnt -/-mice and wild-type mice was analyzed using high-throughput sequencing of bacterial 16S rRNA.Results: In A4gnt -/-mice, which spontaneously develop gastric cancer, the community structure of the gastric microbiome was altered. The relative abundance of mutagenic Desulfovibrio and proinflammatory Prevotellamassilia in these mice was significantly increased, especially 4 weeks after birth. The co-occurrence network appeared to be much more complex. Functional prediction demonstrated considerable decreases in the relative frequencies of functions associated with polysaccharide metabolism and transportation.The distinct profile in A4gnt -/-mice demonstrated a vital role of A4GNT in the establishment of the gastric microbiome. A dysbiotic gastric microbiome may contribute to the spontaneous development of gastric cancer in mice.
Keywords: A4gnt, Gastric microbiome, Desulfovibrio, Prevotellamassilia, gastric cancer
Received: 08 Dec 2024; Accepted: 24 Jan 2025.
Copyright: © 2025 Gong, Gao, Shi, Xu, Yu, Zhang, Wang and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yuqiang Gao, Department of Gastroenterology, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
Rui Shi, Qingdao University Medical College, Qingdao, Shandong Province, China
Xiaona Xu, Central Laboratories, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
Shumin Zhang, Fourth People’s Hospital of Jinan, Jinan, Shandong Province, China
Lili Wang, Central Laboratories, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
Quanjiang Dong, Central Laboratories, Qingdao Municipal Hospital, Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, China
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