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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1541797

ArcB kinase sensor participates in the phagocyte-mediated stress response in Salmonella Typhimurium Salmonella's response to HOCl mediated by ArcB

Provisionally accepted
Coral Pardo-Esté Coral Pardo-Esté 1,2*Francisca Urbina Francisca Urbina 2Nicolas Aviles Nicolas Aviles 2Nicolas Pacheco Nicolas Pacheco 2Alan Cristóbal Briones Alan Cristóbal Briones 2Carolina Elizabeth Cabezas Carolina Elizabeth Cabezas 2Vicente Rojas Vicente Rojas 2Valentina Pavez Valentina Pavez 2Yoelvis Noe Sulbaran Yoelvis Noe Sulbaran 3,4Alejandro A Hidalgo Alejandro A Hidalgo 5Juan Castro-Severyn Juan Castro-Severyn 6Claudia P Saavedra Claudia P Saavedra 2*
  • 1 Laboratorio de Ecología Molecular y Microbiología Aplicada, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad Católica del Norte, Antofagasta, Chile., Antofagasta, Chile
  • 2 Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Santiago Metropolitan Region (RM), Chile
  • 3 Laboratory of Entomology, Institute of Agri-Food, Animal and Environmental Sciences (ICA3), Universidad de O’Higgins, Rancagua 2841959, Chile, Rancagua, Chile
  • 4 Centre of Systems Biology for Crop Protection (BioSaV), Institute of Agri-Food, Animal and Environmental Sciences (ICA3), Universidad de O’Higgins, San Fernando, Chile, San Fernando, Chile
  • 5 Laboratorio de Patogénesis Bacteriana, Facultad de Medicina, Universidad Andres Bello, Santiago, Chile, Santiago, Chile
  • 6 Laboratorio de Microbiología Aplicada y Extremófilos, Departamento de Ingeniería Química, Universidad Católica del Norte,, Antofagasta,, Chile

The final, formatted version of the article will be published soon.

    The ArcAB two component system includes a histidine kinase sensor (ArcB) and a regulator (ArcA) that respond to changes in the cell oxygen availability. The ArcA transcription factor activates genes related to metabolism, membrane permeability and virulence and its presence is required for pathogenicity in S. Typhimurium, which can be phosphorylated independently of its cognate sensor ArcB. In this study we aimed to characterize the transcriptional response to hypochlorous acid (HOCl) mediated by the presence of the ArcB sensor. HOCl is a powerful microbicide widely used for sanitization in industrial settings. We used Salmonella Typhimurium wild type and mutant lacking the arcB gene exposed to NaOCl to describe the global transcriptional response.We also infected murine neutrophils to evaluate the expression level of relevant genes related to the resistance and infection process while facing ROS-related stress. Our results indicate that the absence of the arcB gene significantly affects the ability of S. Typhimurium to grow under HOCl-stress. Overall, 6.6% of Salmonella´s genes varied their expression on the mutant strains, while 8.6% changed in response to NaOCl. The transcriptional response associated with the presence of ArcB is associated with metabolism and virulence, suggesting a critical role in pathogenicity and fitness, especially under ROS-related stress. Our results show that ArcB influences the expression of genes associated with fatty acid degradation, protein secretion, cysteine and H2S biosynthesis and translation both in vitro and under the conditions found inside neutrophils. We found that protein carbonylation is significantly greater on the mutant strain suggesting a critical function of ArcB in the response and repair process. This study contributes

    Keywords: arcB, Sensor kinase, Salmonella, Reactive oxygen stress, HOCl, transcriptomic

    Received: 08 Dec 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Pardo-Esté, Urbina, Aviles, Pacheco, Briones, Cabezas, Rojas, Pavez, Sulbaran, Hidalgo, Castro-Severyn and Saavedra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Coral Pardo-Esté, Laboratorio de Ecología Molecular y Microbiología Aplicada, Departamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad Católica del Norte, Antofagasta, Chile., Antofagasta, Chile
    Claudia P Saavedra, Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Santiago Metropolitan Region (RM), Chile

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