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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1528930
A Comprehensive Analysis of the Genomic and Proteomic Profiles of a Megalocytivirus isolated from Larimichthys crocea
Provisionally accepted
Xiaodong Liu 1,2
Hongshu Chi 1,2* Xixi Yang 1,2* Zaiyu Zheng 1,2* Chunhua Zhu 3*
Yunkun Wu 1,2
Wei-Jen Chang 4* Hui Gong 1,2,5*- 1 Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
- 2 Fujian Engineering Technology Research Center for Aquatic Diseases Control and Prevention, Fuzhou, Fujian Province, China
- 3 Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian Province, China
- 4 Department of Biology, Hamilton College, Clinton, Arkansas, United States
- 5 State Key Laboratory of Mariculture Breeding, Ningde, China
analysis identified 27 viral proteins in the viral-infected cell culture supernatants and 46 viral proteins in the purified virus of FD201807. Among these, 19 viral proteins were detected in both the viral-infected cell culture supernatants and the purified virus samples, while 8 viral proteins were exclusively identified in the viral-infected cell culture supernatants. Notably, there were two proteins derived from the cultured cell line MFF-1 (mandarin fish fry cell line-1), namely cytochrome c and ubiquitin-activating enzyme E1, present in both the purified virus samples and the culture supernatant of infected cells. These cellular proteins may be associated with virus-host protein interactions and/or host cell apoptosis.Discussion: We present the most comprehensive proteomic analysis to date of the megalocytivirus isolated from L. crocea, and help identify highly expressed proteins that may serve as future targets for immunotherapy and biochemical analysis.
Keywords: Genome, Megalocytivirus, Larimichthys crocea, GO analysis, Proteome, Electron microscopy, phylogenetic analysis
Received: 15 Nov 2024; Accepted: 11 Feb 2025.
Copyright: © 2025 Liu, Chi, Yang, Zheng, Zhu, Wu, Chang and Gong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongshu Chi, Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
Xixi Yang, Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
Zaiyu Zheng, Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
Chunhua Zhu, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian Province, China
Wei-Jen Chang, Department of Biology, Hamilton College, Clinton, Arkansas, United States
Hui Gong, Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
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