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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1525887

Bifidobacterium animalis subsp. lactis TG11 Ameliorates Loperamide-Induced Constipation in Mice by Modulating Gut Microbiota

Provisionally accepted
Weiwei Ma Weiwei Ma Yiyang Zhao Yiyang Zhao Yuyang Liu Yuyang Liu Yanyan Wang Yanyan Wang Shuang Yu Shuang Yu Lili Huang Lili Huang *
  • College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China

The final, formatted version of the article will be published soon.

    Probiotics have garnered extensive attention due to their potential health benefits. This study investigates the preventative effects of Bifidobacterium animalis subsp. lactis TG11, derived from the gut of healthy infants, on loperamide-induced constipation in mice. The results indicate that TG11 significantly enhances intestinal motility and maintains stool water content. Additionally, TG11 effectively regulates the levels of gut peptides in the blood, such as motilin (MTL), substance P (SP), somatostatin (SS), endothelin-1 (ET-1), gastrin (Gas), and vasoactive intestinal peptide (VIP). Furthermore, TG11 promotes the production of short-chain fatty acids and improves microbial metabolism. TG11 significantly increased the mRNA expression levels of SCF and c-kit in colonic tissue. Metagenomic sequencing reveals that TG11 improves the composition of the gut microbiota and increases the abundance of beneficial bacteria. In summary, TG11 regulates the intestinal environment and gut health in mice through multiple mechanisms, effectively preventing constipation induced by loperamide.

    Keywords: Probiotics, Constipation, Gut Microbiota, gut peptides, SCF/c-kit

    Received: 12 Nov 2024; Accepted: 10 Jan 2025.

    Copyright: © 2025 Ma, Zhao, Liu, Wang, Yu and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lili Huang, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.