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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiotechnology
Volume 16 - 2025 |
doi: 10.3389/fmicb.2025.1525316
This article is part of the Research Topic Microbial Regulatory Mechanisms in Remediation of Industrial Wastewater and Contaminated Soils View all 8 articles
Stabilization effect and mechanism of heavy metals by microbial consortium of phosphate-solubilizing bacteria and urease-producing bacteria
Provisionally accepted
Xuezhe Zhu 1* Yupin Zhou 1* Zhenghao Yan 2* Yongfang Yan 3* Shuangquan Li 1* Mingjiao Yu 1*
MingJiang Zhang 1,4* Xiao Yan 1*- 1 National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
- 2 Henan University of Science and Technology, Luoyang, Henan Province, China
- 3 Shougang Group talent development Institute, Beijing, China
- 4 Independent researcher, Beijing, China
Stabilization of heavy metals through phosphate-solubilizing bacteria(PSB) induced phosphate precipitation and urease-producing bacteria (UPB) induced carbonate precipitation are promising bioremediation methods. However, little attention has been conducted on the combined action of the above two bioremediations to stabilize heavy metals. Therefore, Microbial consortium of PSB and UPB were constructed for the stabilization of heavy metals by optimizing carbon and nitrogen sources. Results showed that the precipitation of heavy metals could be promoted effectively when soluble starch (10.2 g/L) was used as carbon source and urea (7.8 g/L) as nitrogen source. The stabilization rates for Cu, Zn, Cd and Pb were 98.35%, 99.78%, 99.09% and 92.26% respectively. The stabilization rates of the combined action of PSB and UPB were significantly higher than that of the two microorganisms alone. More indepth analysis of microstructure and composition changes during the biological stabilization process were revealed through the use of XRD, FT-IR, and SEM-EDS.The results showed that the composite metals were precipitated as dense precipitate encased in carbonate and phosphate, and additionally could be stabilized in the form of biosorption. Finally, the stabilization mechanism of heavy metals based on biomineralization and biosorption is proposed. These findings provide new theoretical support for sustainable remediation and management strategies for composite heavy metal polluted areas.
Keywords: Heavy metal contamination, Phosphate-solubilizing bacteria (PSB), Urease-producing bacteria (UPB), Biological stabilization, Biomineralization
Received: 09 Nov 2024; Accepted: 09 Jan 2025.
Copyright: © 2025 Zhu, Zhou, Yan, Yan, Li, Yu, Zhang and Yan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xuezhe Zhu, National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
Yupin Zhou, National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
Zhenghao Yan, Henan University of Science and Technology, Luoyang, 471003, Henan Province, China
Yongfang Yan, Shougang Group talent development Institute, Beijing, China
Shuangquan Li, National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
Mingjiao Yu, National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
MingJiang Zhang, Independent researcher, Beijing, China
Xiao Yan, National Engineering Research Center for Environment-friendly Metallurgy in Producing Premium Non-ferrous Metals, China GRINM Group Corporation Limited, Beijing, China
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