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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1521632
This article is part of the Research Topic Harnessing Microbes for Eco-Friendly Nanoparticle Production and Sustainable Applications View all articles

Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis

Provisionally accepted
Xingyu Gu Xingyu Gu Xiaoju Li Xiaoju Li Ruijia Zhang Ruijia Zhang Ruoli Zheng Ruoli Zheng Mingrui Li Mingrui Li Rong Huang Rong Huang Xin Pang Xin Pang *
  • Shandong University (Qingdao), Qingdao, China

The final, formatted version of the article will be published soon.

    Microorganism-based biosynthesis of quantum dots is a low-cost and green production method with a wide range of potential applications. The development of environmentally friendly synthesis methods is required due to the toxicity and severe reactions that occur during the chemical synthesis of quantum dots. In this study, a novel strain QD4 with the ability to effective and rapid biosynthesis CdS quantum dots is isolated and reported. The isolated strain is a gram-positive, aerobic, flagellated, and rod-shaped bacterium, isolated from seawater. Through the physio-biochemical characterization and 16S rRNA-based phylogenetic tree analysis, the strain is identified as Lysinibacillus boronitolerans QD4. The strain QD4 grows well in the range of 25-40 ℃ (optimum, 37 ℃), pH 5.0-9.0 (optimum, pH 7.0), with a high cadmium-resistance as it could grow at Cd 2+ concentration up to 2 mM, implying its good adaptability to the environment and potential for application. Cd 2+ and Lcysteine are employed as substrates for the biosynthesis of CdS quantum dots by strain QD4. The distinctive yellow fluorescence from CdS quantum dots is visible after only a short induction time (a few hours). Moreover, the properties of the CdS quantum dots are characterized by fluorescence spectroscopy, UV-absorption spectroscopy, TEM, XRD, XPS and infrared spectroscopy. This study provides a novel strain resource for efficient biosynthesis of extracellular, water-soluble quantum dots, paving potential industrial application in green production.

    Keywords: Lysinibacillus boronitolerans, quantum dot, Nanoparticle biosynthesis, Cadmium Sulfide, extracellular

    Received: 02 Nov 2024; Accepted: 03 Jan 2025.

    Copyright: © 2025 Gu, Li, Zhang, Zheng, Li, Huang and Pang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xin Pang, Shandong University (Qingdao), Qingdao, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.