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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Aquatic Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1519074

Whole genome sequencing of a novel carrageenan-degrading bacterium Photobacterium rosenbergii and oligosaccharide preparation

Provisionally accepted
Jing Chen Jing Chen 1,2Runmin Chen Runmin Chen 1Kit-Leong Cheong Kit-Leong Cheong 1Zhuo Wang Zhuo Wang 1Rui Li Rui Li 1Xuejing Jia Xuejing Jia 1Qiaoli Zhao Qiaoli Zhao 1Jianping Chen Jianping Chen 1Xiaofei Liu Xiaofei Liu 1Bingbing Song Bingbing Song 1Saiyi Zhong Saiyi Zhong 1,2*
  • 1 Guangdong Ocean University, Zhanjiang, China
  • 2 Shenzhen Research Institute of Guangdong Ocean University, Shenzhen, China

The final, formatted version of the article will be published soon.

    Carrageenan oligosaccharides are of significant interest due to their diverse bioactivities, necessitating efficient methods for their production. Photobacterium rosenbergii GDSX-4 is a Gramnegative bacterium isolated from the red algae Gracilaria coronopifolia, capable of degrading several polysaccharides. The draft genome was predicted to have 6,407,375bp, 47.55% G+C content and 6,749 genes. Among them, 214 genes encoding carbohydrate enzymes were annotated, including carrageenase, agarose, alginate lyase, and chitinase. Notably, GDSX-4 exhibited exceptional carrageenan-degrading capability, with a specific enzyme activity of 46.94 U/mg. Optimal hydrolysis conditions were identified at 40°C and pH 7.0, with the enzyme retaining 80% of its activity below 30°C and across a pH range of 4.0-10.0. Metal ions such as K⁺, Na⁺, and Ba²⁺ enhanced degradation, while Ni²⁺, Mn²⁺, and Cu²⁺ had inhibitory effects. TLC and ESI-MS analysis revealed that the strain GDSX-4 may hydrolyte kappa-carrageenan into kappa-carrageenan oligosaccharides with degrees of depolymerization of 2-6. These findings highlight the potential of GDSX-4 for the efficient production of carrageenan oligosaccharides, paving the way for applications in the food and agricultural industries.Carrageenan is a macromolecular linear sulfated polysaccharide extracted from red algae, such as Gelidium, Chondrus, and Silvetia (Gurpilhares et al., 2016), and processed into an off-white or light yellow to brownish-yellow powder or granule using water or alkaline solutions. The structure of carrageenan is primarily composed of disaccharide repeating units of α (1,4)-linked D-galactose (D) or 3,6-anhydro-D-galactose residue (DA) and β (1,3)-linked D-galactose residue (G) (Wallace et al., 2020). The three main types of carrageenan, κ-, ι-, and λ-carrageenan, are widely used in various

    Keywords: Photobacterium rosenbergii, Carrageenan, Oligosaccharides, Genome sequencing, preparation

    Received: 29 Oct 2024; Accepted: 02 Jan 2025.

    Copyright: © 2025 Chen, Chen, Cheong, Wang, Li, Jia, Zhao, Chen, Liu, Song and Zhong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Saiyi Zhong, Shenzhen Research Institute of Guangdong Ocean University, Shenzhen, China

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