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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1510058

Transcriptome analysis of 3D4/21 cells expressing CSFV NS4B

Provisionally accepted
Wang Dong Wang Dong 1*Huifang Lv Huifang Lv 1*Yuzhen Song Yuzhen Song 1*Yujin Lv Yujin Lv 1*Xiapeng Xu Xiapeng Xu 2*Huiyuan Jing Huiyuan Jing 1*Zhifeng Peng Zhifeng Peng 1*Xinghui Song Xinghui Song 1*Yongbin Guo Yongbin Guo 1*
  • 1 Henan University of Animal Husbandry and Economy, Zhengzhou, China
  • 2 Agriculture and Rural Affairs Bureau, Dingzhou, China

The final, formatted version of the article will be published soon.

    Classical swine fever (CSF) caused by classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. CSFV non-structural protein 4B (NS4B) plays a crucial role in CSFV replication and pathogenicity. However, the function of NS4B is still limited during CSFV infection. In this study, the RNA-seq was used to investigate differentially expressed genes (DEGs) in 3D4/21 cells expressing CSFV NS4B. 4397 DEGs were identified in 3D4/21 cells expressing NS4B compared to cells expressing the empty vector (NC). 12 DEGs were selected and further verified by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed that these DEGs were associated with endocytosis, autophagy, cell adhesion, transport, immune response, apoptosis and so on. The expression of endocytosis-related genes, including CAV1/2, CAVIN2, Rab1B, CHMP2B/4C, VPS35, SNX2, Rab11B, CHMP6, MVB12B and VPS28, were found to be regulated. In addition, some genes associated with host immune defense, such as USP15, DHX29, DDX3, RIG-I and MDA5, were downregulated and the genes associated with host autophagy, such as WIPI2, ATG16L2, SMCR8, RPTOR and MLST8, were upregulated. Therefore, CSFV NS4B involved in virus invasion and intracellular trafficking, the induction of autophagy and the inhibition of antiviral response. Taken together, this study provides useful information for further understanding the function of NS4B during CSFV infection.

    Keywords: RNA-Seq, Classical swine fever virus, NS4B, Endocytosis, Autophagy

    Received: 12 Oct 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Dong, Lv, Song, Lv, Xu, Jing, Peng, Song and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wang Dong, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Huifang Lv, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Yuzhen Song, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Yujin Lv, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Xiapeng Xu, Agriculture and Rural Affairs Bureau, Dingzhou, China
    Huiyuan Jing, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Zhifeng Peng, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Xinghui Song, Henan University of Animal Husbandry and Economy, Zhengzhou, China
    Yongbin Guo, Henan University of Animal Husbandry and Economy, Zhengzhou, China

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