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METHODS article

Front. Microbiol.
Sec. Microbiotechnology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1500043

Selection and Evaluation of Reference Genes for qRT-PCR in Inonotus obliquus

Provisionally accepted
Lulu Li Lulu Li Xiaofan Guo Xiaofan Guo Shouming Wang Shouming Wang *
  • Hubei Engineering University, Xiaogan, Hubei, China

The final, formatted version of the article will be published soon.

    Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus. Selecting an appropriate reference gene is crucial for researching its gene function. In this study, we selected 11 potential reference genes of Inonotus obliquus. Subsequently, we applied GeNorm, NormFinder, and BestKeeper algorithms to evaluate the expression stability of these 11 reference genes. Under varying carbon sources, VPS exhibited good stability and was suitable as an internal reference gene.Under different nitrogen sources, RPB2 was the most stable reference gene. For varying growth factors, PP2A was the most stable reference gene. At different pH levels, UBQ showed the highest stability. Under different temperature conditions, RPL4 was the most stable. In different strains, RPL2 was the most stable reference gene, while VAS demonstrated the greatest stability across different growth stages. Overall, VPS was the most recommended reference gene for the entire sample set. This study provides a foundation for the further application of RT-qPCR in the gene expression analysis of Inonotus obliquus.

    Keywords: Inonotus obliquus, reference gene, RT-qPCR, Gene Expression, medicinal fungus

    Received: 22 Sep 2024; Accepted: 09 Jan 2025.

    Copyright: © 2025 Li, Guo and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Shouming Wang, Hubei Engineering University, Xiaogan, 432000, Hubei, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.