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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1496701
This article is part of the Research TopicSoil Microbial Communities to Promote Suppressiveness against Soil-Borne Pathogens and DiseasesView all 11 articles
Pandan-vanilla rotation mitigates Fusarium wilt disease in vanilla: insights from rhizosphere microbial community shifts
Provisionally accepted- 1Hainan Academy of Agricultural Sciences, Haikou, China
- 2Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province, China
- 3Nanjing Agricultural University, Nanjing, Jiangsu Province, China
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Vanilla monoculture often leads to Fusarium wilt disease, affecting the industry globally. We evaluated the effects of vanilla-black pepper, -pandan and -sweet rice tea rotations (i.e., growing vanilla in soil previously planted with these crops) on Fusarium oxysporum abundance and rhizosphere microbial communities using real-time quantitative PCR and high-throughput sequencing. Pandan rotation, in particular, reduced disease incidence to 17% and decreased F. oxysporum copy numbers; sweet rice tea showed similar suppressive effects. Crop rotation significantly increased fungal diversity and richness. Different cropping systems, including fallow, monoculture and crop rotation, significantly influenced fungal and bacterial community development, with cropping system and rotated crops being the main drivers of rhizosphere community assembly. The black pepper and pandan rotations specifically enriched certain fungal OTUs, such as OTU1_Thermomyces, OTU37_Arthrobotrys and OTU18_Arthrobotrys, which serve as biomarkers for the presence of F. oxysporum. After pandan rotation, microbial interactions within the rhizosphere intensified, with notable enrichment of core bacterial taxa, including OTU22_Nitrosospira, OTU56_Lacibacterium and OTU178_Actinospica. Soil pH was identified as a significant factor influencing microbial community assembly. The fungal community structure, along with core OTU22_Nitrosospira and soil pH, was pivotal in curbing pathogen growth, explaining 25.19%, 8.61%, and 20.45% of the variance, respectively. This study revealed that incorporating pandan into crop rotation may effectively alleviate soil-borne diseases during vanilla production.
Keywords: Crop rotation, Vanilla, Pandan, microbial community, Rhizosphere microbiome
Received: 15 Sep 2024; Accepted: 16 Apr 2025.
Copyright: © 2025 Hong, Xing, Yang, Zhao, Su, Zhuang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yizhang Xing, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province, China
Qingyun Zhao, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.