Nutraceutical Supplement Slim Reshaped Colon Histomorphology and Reduces Mucispirillum schaedleri in Obese Mice

Freitas, Jessica Alves de; Nehmi Filho, Victor; Santamarina, Aline Boveto; Murata, Gilson Masahiro; Franco, Lucas Augusto Moyses; Fonseca, Joyce Vanessa; Martins, Roberta Cristina; Souza, Gabriele Alves de; Benicio, Gabriela; Sabbag, Isabella Mirandez; de Souza, Esther Alves; Otoch, Jose Pinhata; Pessoa, Ana Flávia Marçal

doi:10.3389/fmicb.2025.1494994

ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Microorganisms in Vertebrate Digestive Systems

Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1494994

This article is part of the Research Topic Harnessing Natural Plant Extracts and Probiotics to Enhance Host-Gut Microbiome Interactions View all 13 articles

Nutraceutical Supplement Slim Reshaped Colon Histomorphology and Reduces Mucispirillum schaedleri in Obese Mice

Provisionally accepted

Jessica Alves de Freitas ^1,2

Victor Nehmi Filho ^1,2

Aline Boveto Santamarina ^1,2

Gilson Masahiro Murata ³

Lucas Augusto Moyses Franco ⁴

Joyce Vanessa Fonseca ⁵

Roberta Cristina Martins ⁴

Gabriele Alves de Souza ⁶

Gabriela Benicio ⁶

Isabella Mirandez Sabbag ⁶

Esther Alves de Souza ^1,2

Jose Pinhata Otoch ^1,2,7

Ana Flávia Marçal Pessoa ^1,2,4*

¹ Laboratório de Produtos e Derivados Naturais, Laboratório de Investigação Médica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Rio Grande do Sul, Brazil
² Pesquisa e Desenvolvimento Efeom Nutrição S/A, São Paulo, Rio Grande do Sul, Brazil
³ Universidade de São Paulo Faculdade de Medicina da Universidade de São Paulo, Departamento de Clínica Médica, Laboratório de Nefrologia (LIM-29), São Paulo, Brazil
⁴ Universidade de São Paulo Instituto de Medicina Tropical de São Paulo, Departamento de Doenças Infecciosas e Parasitárias, Laboratório de Parasitologia Médica (LIM-46), São Paulo, Rio Grande do Sul, Brazil
⁵ Universidade de São Paulo IMT São Paulo, Departamento de Doenças Infecciosas e Parasitárias, Laboratório de Investigação Médica em Protozoologia, Bacteriologia e Resistência Antimicrobiana (LIM-49), São Paulo, Brazil
⁶ Universidade de São Paulo Faculdade de Medicina da Universidade de São Paulo, Departamento de Patologia, Laboratório de Neurociência (LIM-01), São Paulo, Brazil
⁷ Hospital Universitário da Universidade de São Paulo, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil

The final, formatted version of the article will be published soon.

Bioactive compounds and whole foods have emerged as promising interventions to address gut microbiota dysbiosis linked to obesity. Compounds such as berberine and coenzyme Q10 are well-recognized for their roles in managing metabolic syndrome and exerting antioxidant effects, while beet pulp, rich in fiber and antioxidants, enhances gut health through additional prebiotic benefits. This study evaluated the effects of a nutraceutical supplement, Slim, on the modulation of gut microbiota in obese mice induced by a high-fat diet. Our results demonstrated that Slim supplementation significantly improved lipid metabolism, reshaped colon histomorphology, and decreased levels of Mucispirillum schaedleri, which were correlated with VLDL-c and triglycerides.We suggest these effects are driven by a duplibiotic effect, resulting from the synergistic action of the bioactive compounds.

Keywords: nutraceutical, prebiotic, Beet pulp, coenzyme Q10, Berberine, Obesity, Gut Microbiota

Received: 24 Sep 2024; Accepted: 28 Feb 2025.

Copyright: © 2025 Freitas, Nehmi Filho, Santamarina, Murata, Franco, Fonseca, Martins, Souza, Benicio, Sabbag, de Souza, Otoch and Pessoa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Ana Flávia Marçal Pessoa, Laboratório de Produtos e Derivados Naturais, Laboratório de Investigação Médica-26 (LIM-26), Departamento de Cirurgia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Rio Grande do Sul, Brazil

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