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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Phage Biology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1494647
This article is part of the Research Topic The Role of Bacteriophages in Salmonella Diversity, Pathogenicity and Control View all 6 articles

Isolation, characterization and liposome-loaded encapsulation of a novel virulent Salmonella phage vB-SeS-01

Provisionally accepted
Yuhang Luo Yuhang Luo 1Jacques Mahillon Jacques Mahillon 2Lin Sun Lin Sun 1Ziqiong You Ziqiong You 1Xiaomin Hu Xiaomin Hu 1*
  • 1 College of Life Sciences, South-Central Minzu University, Wuhan, China
  • 2 Laboratory of Food and Environmental Microbiology, Université catholique de Louvain, Louvain-la-Neuve, Belgium

The final, formatted version of the article will be published soon.

    This study characterized vB-SeS-01, a novel Guernseyvirinae phage preying on Salmonella enterica, whose genome is closely related to those of phages SHWT1 and vB-SenS-EnJE1. Phage vB-SeS-01 displays strong lysis ability against 9 out of 24 tested S. enterica strains (including the pathogenic "Sendai" and "Enteritidis" serovars), high replicability with a burst size of 111  15 PFU/ cell and a titre up to 2.1 × 10 11 PFU/mL, and broad pH (4.0~13.0) and temperature (4~80 °C) stabilities. Furthermore, nine vB-SeS-01 liposome-carrying formulations were prepared, of which the one encapsulated with PC:Chol:T80:SA = 9:1:2:0.5 without sonication displayed the optimal features. This formulation carried up to 10 11 PFU/mL, with an encapsulation rate of 80%, an average size of 172.8 nm, and a polydispersity index (PDI) of 0.087. It remained stable at 4℃ and 23°C for at least 21 days and at 37℃ for 7 days. Both vB-SeS-01 and vB-SeS-01-loaded liposomes displayed intracellular antimicrobial effects and could reduce the transcription level of some tested intracellular inflammatory factors caused by the infected S. enterica sv. Sendai 16226 and Enteritidis 50041CMCC.

    Keywords: Salmonella enterica, Phage, Guernseyvirinae, Liposome, intracellular antibacterial effect

    Received: 11 Sep 2024; Accepted: 09 Jan 2025.

    Copyright: © 2025 Luo, Mahillon, Sun, You and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xiaomin Hu, College of Life Sciences, South-Central Minzu University, Wuhan, China

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