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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1470731
This article is part of the Research Topic Gut Microbiota Modulation to Mitigate Stress-Induced Functional Changes View all 11 articles
Integrated metabolomics and intestinal microbiota approach to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
Provisionally accepted
Dongbo Li 1 Chao Li 1 Ning Liu 2 Hanzhong Liu 2 Zhiju Yu 2 Quanjin Liu 1
Gang Shu 1 Juchun Lin 1 Wei Zhang 1
Guangneng Peng 1 Ling Zhao 1 Huaqiao Tang 1 Haohuan Li 1 Funeng Xu 1
Hualin Fu 1*- 1 Sichuan Agricultural University, Ya'an, China
- 2 Sichuan Academy of Grassland Science, Chengdu Institute of Biology, Chinese Academy of Sciences (CAS), Chengdu, Sichuan Province, China
Post-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death in severe cases. Traditional Chinese medicine (TCM) has been widely used in animal due to its advantages of natural origin, diverse functions, safety, reliability, economy and environmental protection. Modified Yupingfeng Granule (MYPFG) is an improved Yupingfeng prescription based on the famous traditional Chinese prescription Yupingfeng (YPF), which is combined with other TCM and has obvious synergistic and additive activity in order to obtain an excellent natural medicine for PWD. In this study, 120 weaned Rex rabbits were randomly allocated to 4 treatment groups, including control (CON), low dose (LD), medium dose (MD), high dose (HD). Rabbits were fed a control diet or a different MYPFG proportions of diet for 30 days. Results showed that MYPFG increased average daily gain, villus length to crypt depth ratio and decreased the feed to meat ratio, diarrhea frequency, mortality rate, depth of crypt (P<0.05). The study also combined 16S rRNA analysis of intestinalgut microbiota and cecal contents metabolomics to explore the MYPFG effect on weaned Rex rabbits. The intestinal microbiotaflora test found that MYPFG could change the abundances of Patescibacteria, Sphingobium, Ruminococcus and Oxalobacter. Metabolomics analysis found that effect may be related to its regulation of Glycine, serine and threonine metabolism, Arginine and proline metabolism. Nicotinate and nicotinamide metabolism. MYPFG could regulate intestinal microbiotaflora and change the metabolic pathway of some amino acids to alleviate the PWD in Rex rabbits.
Keywords: Modified Yupingfeng Granule, post-weaning diarrhea, intestinal microbiota, Metabolomics PWD, Post-weaning Diarrhea, TCM, traditional Chinese medicine, YPFP, Yupingfeng Powder, MYPFG, Modified Yupingfeng Granule, HPLC, high-performance liquid chromatography
Received: 26 Jul 2024; Accepted: 17 Mar 2025.
Copyright: © 2025 Li, Li, Liu, Liu, Yu, Liu, Shu, Lin, Zhang, Peng, Zhao, Tang, Li, Xu and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hualin Fu, Sichuan Agricultural University, Ya'an, China
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