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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1534761
This article is part of the Research Topic Soil Biodiversity and Regenerative Agriculture: The Path to Achieve SDGs View all articles

Relevance of Plant growth-promoting bacteria (PGPB) in reducing the severity of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici by altering metabolites and related genes

Provisionally accepted
Waquar Akhter Ansari Waquar Akhter Ansari 1*Ram Krishna Ram Krishna 2SARVESH PRATAP KASHYAP SARVESH PRATAP KASHYAP 3Khalid Alanzi Khalid Alanzi 4Mohammad Abul Farah Mohammad Abul Farah 4Durgesh K Jaiswal Durgesh K Jaiswal 5Akhilesh Yadav Akhilesh Yadav 6Mohammad Tarique Zeyad Mohammad Tarique Zeyad 7JAY PRAKASH VERMA JAY PRAKASH VERMA 2
  • 1 Marwadi University, Rajkot, Gujarat, India
  • 2 Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, Uttar Pradesh, India
  • 3 bio zenith, New Delhi, India
  • 4 King Saud University, Riyadh, Riyadh, Saudi Arabia
  • 5 Graphic Era University, Dehradun, Uttarakhand, India
  • 6 University of California, Los Angeles, California, United States
  • 7 Aligarh Muslim University, Aligarh, Uttar Pradesh, India

The final, formatted version of the article will be published soon.

    Among the biotic stresses wilt disease severely affects tomato quality and productivity globally, the causal organism for this disease is Fusarium oxysporum f. sp. lycopersici (Fol), which is very well-known and it has a significant effect on the productivity of other crops also. Concern has been taken to find out the effect of Plant Growth Promoting Bacteria (PGPB) on tomato wilt disease alleviation. Four PGPB viz; Pseudomonas aeruginosa BHUPSB01 (T1), Pseudomonas putida BHUPSB04 (T2), Paenibacillus polymyxa BHUPSB16 (T3), and Bacillus cereus IESDJP-V4 (T4), were used as inocula to treat Fol challenged plants. The results reveal that PGPB treatments T1, T2, T3, and T4 were able to decrease the severity of Fusarium wilt on tomato plants at different levels. Among the treatments, (T3) displayed the most protective defense, with the lowest disease frequency which was 15.25%. There were no significant differences observed in parameters like fruit yield and relative water contents in PGPBinoculated plants, although T3, and T4 showed minimal electrolyte leakage. Significant changes in Chlorophyll fluorescence were also recorded. The lower level of H2O2 and malondialdehyde (MDA) was recorded in T3 and T4 treatments. Additionally, proline accumulation was highest in T3-treated plants. Antioxidative enzyme activities, such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) significantly increased in PGPB treated. Furthermore, the highest phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) activity was reported in T3 and T4 plants, respectively. PGPB treated plants showed elevated expression of PAL, PPO, PR3, PR2, SOD, CAT, and PO, genes. This study's results reveal that PGPB strains can be utilized as biocontrol agents to enhance tomato resistance against Fusarium wilt.

    Keywords: Fusarium Wilt, Tomato, PGPB, Bio-priming, Antioxidative enzyme, gene expression analysis

    Received: 26 Nov 2024; Accepted: 30 Dec 2024.

    Copyright: © 2024 Ansari, Krishna, KASHYAP, Alanzi, Abul Farah, Jaiswal, Yadav, Zeyad and VERMA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Waquar Akhter Ansari, Marwadi University, Rajkot, 360003, Gujarat, India

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