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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1529351
Characterization and pathogenicity of a novel avian orthoreovirus in China
Provisionally accepted
Shunyan Chen
1*
Jialin Yang
1*
Li Li
1*
Yawei Guo
1*
Shenghua Yang
2*
Zetao Su
1*
Sucan Zhao
1*
Li Xue-song
2
Wencheng Lin
1
Yunping Du
2*
Lijuan Yin
2*
Lianxiang Wang
2*
Feng Chen
1*- 1 College of Animal Science, South China Agricultural University, Guangzhou, China
- 2 Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, China
Avian orthoreovirus (ARV) is a significant etiological agent responsible for viral arthritis, causing great economic losses in the global poultry industry. In this study, we isolated and fully sequenced a novel ARV strain, designated FJ202311, from a poultry farm in Fujian province, China. Using next-generation sequencing with MGI technology, we determined that the genome of strain FJ202311 is approximately 23,495 base pairs in length and encodes 12 major proteins. Phylogenetic analysis of the sigma C amino acid sequence revealed that FJ202311 forms a distinct genotypic cluster, with only 47.1% to 59.3% sequence identity to 16 reference ARV strains.Detailed sequence analysis identified 50 unique amino acid substitutions within the sigma C protein of the FJ202311 strain. Pathogenicity studies indicated that FJ202311 induces severe arthritis and tenosynovitis in broilers. Infected chickens exhibited significant weight loss compared to the control group, with reductions of 11.78% and 8.93% at 14 and 21 days post-infection, respectively. These findings advance our understanding of ARV molecular epidemiology in China and emphasize the unique characteristics of this newly identified strain. The discovery of FJ202311 underscores the necessity for ongoing surveillance and could guide the development of improved ARV prevention and control strategies.
Keywords: Avian orthoreovirus, Isolation, Genomic Analysis, phylogenetic analysis, pathogenicity
Received: 16 Nov 2024; Accepted: 24 Dec 2024.
Copyright: © 2024 Chen, Yang, Li, Guo, Yang, Su, Zhao, Xue-song, Lin, Du, Yin, Wang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Shunyan Chen, College of Animal Science, South China Agricultural University, Guangzhou, China
Jialin Yang, College of Animal Science, South China Agricultural University, Guangzhou, China
Li Li, College of Animal Science, South China Agricultural University, Guangzhou, China
Yawei Guo, College of Animal Science, South China Agricultural University, Guangzhou, China
Shenghua Yang, Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, China
Zetao Su, College of Animal Science, South China Agricultural University, Guangzhou, China
Sucan Zhao, College of Animal Science, South China Agricultural University, Guangzhou, China
Yunping Du, Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, China
Lijuan Yin, Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, China
Lianxiang Wang, Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu, China
Feng Chen, College of Animal Science, South China Agricultural University, Guangzhou, China
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