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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Symbioses
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1527512
This article is part of the Research Topic Plant Microbiome: Interactions, Mechanisms of Action, and Applications, Volume III View all 18 articles

Effects of biological and abiotic factors on dark septate endophytes distribution and heavy metal resistance in different environments

Provisionally accepted
Zhenzhou Wang Zhenzhou Wang Wenyi Shi Wenyi Shi Xiuling Chen Xiuling Chen Yuli Lin Yuli Lin Wenjing Chen Wenjing Chen Li Yao Li Yao Xiang Sun Xiang Sun Xia Li Xia Li *Xueli He Xueli He *
  • Hebei University, Baoding, China

The final, formatted version of the article will be published soon.

    Exploring the interactions between dark septate endophytes (DSE) in plant roots across diverse heavy metal habitats-considering host plants, site characteristics, and microbial communities-provides insights into the distribution patterns of DSE in metal-rich environments and their mechanisms for developing heavy metal resistance.This study collected samples of three common plant species (Phragmites australis, PA, Setaria viridis, SV, and Artemisia annua, AA) and their corresponding soil samples from three heavy metal-contaminated sites: Baiyang Lake, BY, Fengfeng mining area, FF, and Huangdao, HD. Utilizing high-throughput sequencing and physicochemical analysis methods, the biological and abiotic factors affecting DSE colonization and distribution in the roots were investigated. Twenty-two DSE species were isolated and identified with 11 species found in BY, 8 species in FF, and 11 species in HD. The screening for heavy metal resistance discovered 10 heavy metal-tolerant DSE species.Soil available phosphate, available nitrogen, and Cd contents, as well as the composition of different root fungal communities, influence the resistance and distribution of heavy metal-tolerant DSE. Notably, 12 DSE species, including Paraphoma radicina and Paraphoma chrysanthemicola, were reported for the first time in heavy metal habitats. The colonization rates of DSE in the roots of PA (96%) and AA (76%) were highest in BY, while the highest colonization rate in the roots of SV was observed in HD (94%). Site-specific soil parameters, such as available K, organic contents, Zn, and Cd contents from different sites are the main determinants affecting DSE colonization. Meanwhile, the diversity and richness of other root-associated endophytic fungi, which varied considerably across sites, served as significant biological factors influencing DSE distribution and colonization. The results of this study provide a strong theoretical framework for the effective utilization of DSE fungi to mitigate soil heavy metal pollution.

    Keywords: Dark septate endophytes, distribution, Fungal diversity, heavy metal habitats, Heavy metal resistance

    Received: 13 Nov 2024; Accepted: 23 Dec 2024.

    Copyright: © 2024 Wang, Shi, Chen, Lin, Chen, Yao, Sun, Li and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xia Li, Hebei University, Baoding, China
    Xueli He, Hebei University, Baoding, China

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