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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Phage Biology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1525040
This article is part of the Research Topic Innovation in Tackling the Global Challenge of Eradicating Antibiotic-Resistant Microorganisms View all 4 articles

Bacteriophage LDT325 Enhances Pseudomonas syringae Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis ( L.) O. Kuntze)

Provisionally accepted
Li Liu Li Liu Anqi Huang Anqi Huang Hua Zhang Hua Zhang Yubao Li Yubao Li Lei Wang Lei Wang *
  • Liaocheng University, Liaocheng, China

The final, formatted version of the article will be published soon.

    Bud blight caused by Pseudomonas syringae is a serious disease affecting tea plants and causing severe damage to production output and quality. Phages play an important role in controlling the development of bacterial diseases in plants. Previous studies have shown that the tolerance of phage-treated tea plants to bud blight was notably greater compared with that of the control group. In the present study, we determined the effect of bacteriophage therapy on physiological and biochemical parameters of tea leaves. Transmission electron microscopy (TEM) was used to analyze the cellular structure of tea leaves, and bioinformatics was used to analyze the phage. Results revealed that bacteriophage treatment can enhance the expression of antioxidant enzyme genes (CsSOD, CsCAT, and CsPOD). The levels of osmotic adjustment compounds, including proline and soluble sugars, were also elevated, suggesting that bacteriophage enhances the osmotic adjustment capacity in tea plants. TEM analysis revealed that the integrity of the cell structure of the tea leaves treated with phage was notably better compared with that of the control group. Interestingly, we also observed that the phage lysed the animal pathogen Salmonella as well as the plant pathogen P. syringae. Using NCBI BLASTn to compare the entire genome with other nucleotide sequences, we found that the phage LDT325 exhibited cross-species characteristics that had not been previously reported. In summary, our findings demonstrate that bacteriophages can protect tea plants from damage caused by bacterial diseases by regulating antioxidant systems.

    Keywords: Camellia sinensis, Pseudomonas syringae, Bacteriophage, Physiological characters, antioxidant enzyme

    Received: 08 Nov 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Liu, Huang, Zhang, Li and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lei Wang, Liaocheng University, Liaocheng, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.