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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1524888
This article is part of the Research Topic Diagnostic Innovations for Microbial Pathogens in Edible Plants: Cutting-Edge Technologies for Enhanced Detection View all articles
Plant growth promotion via priming with volatile organic compounds emitted from Bacillus vallismortis strain EXTN-1
Provisionally accepted
Swarnalee Dutta
1*
Kotnala Balaraju
2*
Soh-Young Oh
3*
Mi-Hyun Lee
2
Se Weon Lee
2*
Yong Hwan Lee
2*
Kyungseok Park
4*- 1 Division of Biotechnology, Jeonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596, Republic of Korea, Iksani, Republic of Korea
- 2 Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea, Wanju,, Republic of Korea
- 3 Research Institute of International Agriculture, Technology and Information, Hankyong National University, Ansung, 17579, Republic of Korea, Ansung, Republic of Korea
- 4 Hankyong National University, Anseong, Republic of Korea
Volatile organic compounds (VOCs) produced by potential plant growth-promoting rhizobacteria (PGPR) play an important role in plant interactions. However, the mechanisms underlying this phenomenon are not well understood. Our findings show that the influence of VOCs from the PGPR strain Bacillus vallismortis (EXTN-1) on tobacco plant growth is dependent on the culture media used. The VOCs released from sugar-rich media such as Deleted: 33 2 potato dextrose agar (PDA) and King's B (KB) media were highly effective. However, exposure to VOCs from nutrient agar (NA), tryptic soy agar (TSA), and Luria-Bertani (LB) resulted in chlorosis and stunted plant growth. This effect was caused by the discharge of a large amount of ammonia that altered the pH of the plant growth media. Seedlings exposed to VOCs for 10 days exhibited improved growth even after the VOCs were eliminated under greenhouse conditions. Priming of seeds with VOCs for 24 and 48 h induced higher growth than the untreated control, and seeds with 48 h exposure were better as compared to 24 h treatment. Chemical characterization of VOCs emitted by EXTN-1 in different media using solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) showed the presence of 2,3-butanedione and monoxime in all spectra. However, 1butanol was the prominent peak in VOC of EXTN-1 grown in KB and NA, while acetoin was highest in PDA, followed by KB. Heneicosane and benzaldehyde were exclusively produced in NA media, and these synthetic compounds improved growth in the I-plate assay. This work indicates that VOCs released from EXTN-1 are important for the growth-promoting effect of EXTN-1.
Keywords: Bacillus vallismortis, VOCs, Heneicosane, induced systemic resistance, Seed priming, I-plate assay
Received: 08 Nov 2024; Accepted: 23 Dec 2024.
Copyright: © 2024 Dutta, Balaraju, Oh, Lee, Lee, Lee and Park. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Swarnalee Dutta, Division of Biotechnology, Jeonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596, Republic of Korea, Iksani, Republic of Korea
Kotnala Balaraju, Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea, Wanju,, Republic of Korea
Soh-Young Oh, Research Institute of International Agriculture, Technology and Information, Hankyong National University, Ansung, 17579, Republic of Korea, Ansung, Republic of Korea
Se Weon Lee, Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea, Wanju,, Republic of Korea
Yong Hwan Lee, Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea, Wanju,, Republic of Korea
Kyungseok Park, Hankyong National University, Anseong, Republic of Korea
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