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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1512027

Effect of Potato Glycoside Alkaloids on Mitochondria Energy Metabolism of Fusarium solani, the Root Rot Pathogen of Lycium Barbarum

Provisionally accepted
Ruiyun Li Ruiyun Li Bin Wang Bin Wang Wei Chen Wei Chen Chongqing Zhang Chongqing Zhang Nan Li Nan Li Yupeng Wang Yupeng Wang Yuke Yan Yuke Yan Yuyan Sun Yuyan Sun Jing He Jing He *
  • College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province, China

The final, formatted version of the article will be published soon.

    This study employed tandem mass labeling (TMT) quantitative proteomics technology to investigate the antifungal mechanism of potato glycoside alkaloids (PGA) against Fusarium solani. We elucidated the antifungal mechanism of PGA from the perspective of mitochondrial proteome molecular biology. Furthermore, we identified and annotated the differentially expressed proteins (DEP) of F. solani under PGA stress. A total of 2412 DEPs were identified, among which 1083 were significantly up-regulated and 1329 significantly down-regulated. Subsequent analysis focused on five DEPs related to energy metabolism for verification at both protein and gene levels. Gene Ontology (GO) function analysis revealed that the DEPs were primarily involved in the integral component of the membrane, intrinsic component of the membrane, pyridine-containing compound metabolic processes, carbon-oxygen lyase activity, and the endoplasmic reticulum, with a notable enrichment in membrane components. Furthermore, a total of 195 pathways were identified through KEGG analysis, with significant enrichment in critical pathways including pentose and glucuronate interconversions, propanoate metabolism, various types of N-glycan biosynthesis, the pentose phosphate pathway, and carbon fixation in photosynthetic organisms. The results from both parallel reaction monitoring (PRM) and real-time RT-qPCR were consistent with the overall trends observed in TMT proteomics, thereby confirming the validity of the TMT proteomics analysis. These findings indicate that PGA inhibits the growth of F. solani by impacting mitochondrial energy metabolism.

    Keywords: Fusarium solani, TMT technology, Potato glycoside alkaloids, Energy Metabolism, mitochondrion

    Received: 16 Oct 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Li, Wang, Chen, Zhang, Li, Wang, Yan, Sun and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jing He, College of Forestry, Gansu Agricultural University, Lanzhou, 730070, Gansu Province, China

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