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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1501373
This article is part of the Research Topic Women in Infectious Agents and Disease: 2024 View all articles

Comparative analysis of antimicrobial resistance and genetic diversity of Bordetella bronchiseptica isolates obtained from swine within the United States

Provisionally accepted
Tracy L Nicholson Tracy L Nicholson *Sarah M Shore Sarah M Shore
  • National Animal Disease Center, Agricultural Research Service (USDA), Ames, United States

The final, formatted version of the article will be published soon.

    Bordetella bronchiseptica is bacterial pathogen that is pervasive in swine populations and serves multiple roles in respiratory disease. The goals of this study were to use whole-genome sequencing (WGS) analysis to assess the sequence type (ST), identify the genetic diversity of genes predicted to encode regulatory and virulence factors, and evaluate any potential antimicrobial resistance harbored by B. bronchiseptica isolates obtained from swine within the U.S. While a generally high degree of genomic conservation was observed among the swine B. bronchiseptica isolates, genetic diversity was identified within the fimNX locus and among the sequence type six (ST6) isolates. The majority of B. bronchiseptica isolates exhibited phenotypic resistance to four antibiotic classes, however, only three antimicrobial resistance genes were identified. Combined the data suggests that B. bronchiseptica isolates are not serving as a source of antimicrobial resistance gene transference in the swine production environment.

    Keywords: Bordetella bronchiseptica, Whole-genome sequencing (WGS), Comparative genomics, virulence factor, Average Nucleotide Identity (ANI), Sequence type (ST), Antimicrobial resistance (AMR), Mobile genetic element (MGE)

    Received: 24 Sep 2024; Accepted: 04 Nov 2024.

    Copyright: © 2024 Nicholson and Shore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tracy L Nicholson, National Animal Disease Center, Agricultural Research Service (USDA), Ames, United States

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