Shuangyuan Liu
1
Yaqi Liu
2
Jingcheng Dai
3*
Dongru Qiu
2*The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1498681
This article is part of the Research Topic New Insight into the Roles of Microorganisms in Municipal and Environmental Engineering Technologies/Systems - Volume II View all articles
Comparative analyses on Nitrogen Removal Microbes and Functional Genes within Anaerobic-Anoxic-Oxic (A 2 /O) and Deoxidation Ditch Sewage-treating Processes in Wuhan and Xi'an Cities, China
Provisionally accepted- 1 Eco-Environmental Monitoring and Research Center, Pearl River Valley and South China Sea Ecology and Environment Administration, Ministry of Ecology and Environment, Guangzhou, China
- 2 Institute of Hydrobiology, Chinese Academy of Sciences (CAS), Wuhan, Hubei Province, China
- 3 School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China
- 4 Ecological Environment Science and Technology Center, Wuhan, China
Introduction: Anaerobic-anoxic-oxic (A 2 /O) and deoxidation ditch (DOD) processes are being increasingly preferred owing to their effectiveness in treating various wastes in wastewater treatment plants (WWTPs). Considering seasonal variations is crucial in optimizing treatment processes, ensuring compliance with regulations, and maintaining the overall efficiency and effectiveness of WWTPs. This study aimed to determine the influence of seasonality on nitrogen removing microbes and functional genes within A 2 /O and DOD processes in the humid Wuhan and semi-arid Xi'an cities, China.The physicochemical parameters of water quality were determined, and molecular and bioinformatic analyses of the bacterial community and nitrogen metabolism functional genes in the two different treatment processes of two WWTPs were performed over four seasons.Results and Discussion: Our analyses revealed a significant difference in all physicochemical parameters across all experimental groups (P < 0.05). At the genus level, the abundance of Dokdonella, one unidentified genus of Nitrospiraceae, Terrimonas, and one unidentified genus of Chloroflexi was the highest in all groups. Generally, warmer seasons exhibited higher biodiversity indices. The A 2 /O system exhibited higher values in terms of most nitrogen metabolism functional genes than those of the DOD sewage treatment system. In both WWTPs, the abundance of most genes in spring and summer were higher than that of autumn and winter seasons. Taken together, changes in temperature, caused by seasonal changes, may contribute to changes in abundance of nitrogen metabolic functional genes.
Keywords: Anaerobic-anoxic-oxic system, Deoxidation ditch process, Nitrogen metabolism genes, Seasonal variation, wastewater treatment
Received: 19 Sep 2024; Accepted: 16 Oct 2024.
Copyright: © 2024 Liu, Liu, Ye, Xiao, Xu, Dai and Qiu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jingcheng Dai, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China
Dongru Qiu, Institute of Hydrobiology, Chinese Academy of Sciences (CAS), Wuhan, Hubei Province, China
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