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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1498653
This article is part of the Research Topic Recent Advances in Agricultural Waste Recycling by Microorganisms and Their Symbiosis View all 7 articles

Whole-genome Sequencing and Secondary Metabolite Exploration of the Novel Bacillus velezensis BN with Broad-spectrum Antagonistic Activity Against Fungal Plant Pathogens

Provisionally accepted
Yanli Zheng Yanli Zheng 1,2*Tongshu Liu Tongshu Liu 2*Xu Wang Xu Wang 2*Haiyan Wang Haiyan Wang 2*Ying Li Ying Li 2*Wangshan Zheng Wangshan Zheng 1,2*Jiajia Li Jiajia Li 1,2*Yan Yang Yan Yang 2Yang Liu Yang Liu 2*Zhaoyu Li Zhaoyu Li 2*Qiang Wang Qiang Wang 3Yongqiang Tian Yongqiang Tian 2*
  • 1 Lanzhou Jiaotong University, Lanzhou, China
  • 2 School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
  • 3 State Key Laboratory of Crop Adversity Adaptation and Improvement, College of Life Science, Henan University, Kaifeng, Henan Province, China

The final, formatted version of the article will be published soon.

    The utilization of chemical pesticides recovers 30-40% of food losses. However, their application has also triggered a series of problems, including food safety, environmental pollution, pesticide resistance, and incidents of poisoning. Consequently, green pesticides are increasingly seen as viable alternatives to their chemical counterparts. Among these, Plant Growth-Promoting Rhizobacteria (PGPR), which are found within plant root systems, stand out for their capacity to stimulate plant growth. Recently, we isolated a strain, BN, with broad-spectrum antimicrobial activity from the rhizosphere of Lilium brownii. Identification revealed that this strain belongs to the species Bacillus velezensis and exhibits significant inhibitory effects against various fungal plant pathogens. The complete genome sequence of B. velezensis BN consists of a circular chromosome with a length of 3,929,791 bp, includes 3,747 protein-coding genes, 81 small RNAs, 27 rRNAs, and 86 tRNAs. Genomic analysis revealed that 29% of the genes are directly involved in plant growth, while 70% of the genes are indirectly involved. In addition, 12 putative biosynthetic gene clusters were identified, responsible for the synthesis of secondary metabolites such as non-ribosomal peptides, lanthipeptides, polyketides, siderophores, and terpenes. These findings provide a scientific basis for the development of efficient antimicrobial agents and the construction of biopesticide production platforms in chassis cells.

    Keywords: Green pesticides, Food Safety, Plant growth-promoting rhizobacteria (PGPR), Antimicrobial agents, Non-ribosomal peptides

    Received: 19 Sep 2024; Accepted: 08 Nov 2024.

    Copyright: © 2024 Zheng, Liu, Wang, Wang, Li, Zheng, Li, Yang, Liu, Li, Wang and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yanli Zheng, Lanzhou Jiaotong University, Lanzhou, China
    Tongshu Liu, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Xu Wang, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Haiyan Wang, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Ying Li, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Wangshan Zheng, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Jiajia Li, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Yang Liu, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Zhaoyu Li, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China
    Yongqiang Tian, School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, Gansu, China, Lanzhou, China

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