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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Food Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1498540
This article is part of the Research Topic Probiotics for Global Health: Advances, Applications and Challenges View all 10 articles

Weizmannia coagulans BC99 Alleviates Hyperuricemia and Oxidative Stress via DAF-16/SKN-1 Activation in Caenorhabditis elegan

Provisionally accepted
Gao Yinyin Gao Yinyin 1Li Cheng Li Cheng 2Li Junfei Li Junfei 2Duan Mengyao Duan Mengyao 2Li Xuan Li Xuan 2Zhao Lina Zhao Lina 2Wu Ying Wu Ying 2Gu Shaobin Gu Shaobin 2*
  • 1 Henan University of Science and Technology, Luoyang, Henan Province, China
  • 2 Henan University of Science and Technology, Luoyang, China

The final, formatted version of the article will be published soon.

    This study established a hyperuricemia model using Caenorhabditis elegans (C. elegans), and studied the anti-hyperuricemia activity and potential mechanisms of Weizmannella coagulans BC99 (W. coagulans) at different concentrations (107 CFU/mL BC99, 108 CFU/mL BC99). Subsequently, we utilized UPLC-Q-TOF/MS to investigate the impact of BC99 on endogenous metabolites in C. elegans and identified pathways and biomarkers through differential metabolomics analysis. The results of this study showed that BC99 treatment significantly reduced the expression of P151.2 and T22F3.3 (p < 0.05), reduced the levels of uric acid (UA) and xanthine oxidase (XOD) in nematodes (p < 0.05), while extending their lifespan and movement ability (p < 0.05). Mechanistically, BC99 activates the transcription factors DAF-16 and SKN-1, thereby inducing the expression of stress response genes, enhancing the activity of antioxidant enzymes and tolerance to heat stress in the body, and reducing the production of ROS (p < 0.001). This effect was most significant in the H-BC99 group. Furthermore, non-targeted metabolomics indicated that BC99 predominantly regulated pathways associated with amino acid metabolism (Carnosine), glycerophospholipid metabolism, and purine metabolism. These results underscore BC99 as an effective and economical adjunct therapeutic agent for hyperuricemia, providing a scientific basis for further development and application.

    Keywords: Weizmannella coagulans, Caenorhabditis elegans, Hyperuricemia, Metabolomics, Mechanism

    Received: 19 Sep 2024; Accepted: 02 Dec 2024.

    Copyright: © 2024 Yinyin, Cheng, Junfei, Mengyao, Xuan, Lina, Ying and Shaobin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gu Shaobin, Henan University of Science and Technology, Luoyang, China

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