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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1493766

Analysis of the Effects of Bacillus velezensis HJ-16 Inoculation on Tobacco Leaves Based on Multi-omics Methods

Provisionally accepted
Zhou Qing Zhou Qing 1,2Yang J. chu Yang J. chu 1*Feng Y. jie Feng Y. jie 1*Yang Z. can Yang Z. can 1*Wang Y. xuan Wang Y. xuan 1*Zhang Zhan Zhang Zhan 1*Zhang T. ting Zhang T. ting 1Liu W. zhao Liu W. zhao 1*Xu Y. ming Xu Y. ming 1*Yang Y. feng Yang Y. feng 1*Huang J. hong Huang J. hong 2*
  • 1 China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan Province, China
  • 2 Henan University, Kaifeng, China

The final, formatted version of the article will be published soon.

    In this study, a strain isolated from the surface of flue-cured tobacco leaves, identified as Bacillus velezensis HJ-16, was applied in the solid-state fermentation of tobacco leaves. This strain, known for producing thermally stable enzymes, including amylase, cellulase, and protease, significantly improved the sensory qualities of tobacco, enhancing aromatic intensity, density, and softness, while reducing irritation. Whole-genome sequencing and functional annotation revealed that B. velezensis HJ-16 possesses a single circular chromosome containing genes associated with enzyme production and metabolic activities, particularly in carbohydrate metabolism and amino acid metabolism. Untargeted metabolomics analysis identified significant changes in non-volatile metabolites induced by fermentation. These metabolites were enriched in pathways related to flavonoid biosynthesis, alkaloid biosynthesis, aromatic amino acid metabolism, lipid metabolism, and carbon metabolism. Metagenomic analysis showed that Bacillus became the dominant genus on the tobacco leaf surface following inoculation with B. velezensis HJ-16, altering the microbial community composition, reducing diversity and evenness, and enhancing microbial metabolic activity. These findings underscore the potential of B. velezensis HJ-16 as a biotechnological tool to improve tobacco leaf quality.

    Keywords: Tobacco, Bacillus velezensis, Fermentation, Whole-genome sequencing, Metagenomic, untargeted metabolomics

    Received: 09 Sep 2024; Accepted: 06 Nov 2024.

    Copyright: © 2024 Qing, chu, jie, can, xuan, Zhan, ting, zhao, ming, feng and hong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yang J. chu, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Feng Y. jie, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Yang Z. can, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Wang Y. xuan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Zhang Zhan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Liu W. zhao, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Xu Y. ming, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Yang Y. feng, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Huang J. hong, Henan University, Kaifeng, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.