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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1493621
This article is part of the Research Topic Insights in Infectious Agents and Disease: 2023/2024 View all 27 articles

Development of a Providencia stuartii MultiLocus Sequence Typing scheme

Provisionally accepted
Gabriele Arcari Gabriele Arcari 1,2*Alice De Francesco Alice De Francesco 3,4Riccardo Polani Riccardo Polani 3Alessandra Carattoli Alessandra Carattoli 3Valerio Capitani Valerio Capitani 5
  • 1 University of Insubria, Varese, Italy
  • 2 Department of Medicine and Technological Innovation, University of Insubria, Varese, Italy
  • 3 Department of Molecular Medicine, Faculty of Pharmacy and Medicine, Sapienza University of Rome, Rome, Lazio, Italy
  • 4 ASST Sette Laghi, Varese, Italy
  • 5 Department of Public Health and Infectious Diseases, Faculty of Pharmacy and Medicine, Sapienza University of Rome, Rome, Lazio, Italy

The final, formatted version of the article will be published soon.

    The Providencia genus is assuming greater clinical relevance among infections caused by Enterobacterales also because of its intrinsic and acquired resistance to last-resort antibiotics. However, despite having been known and studied for over 50 years, genomics and taxonomy of the Providencia genus are currently undergoing a deep rearrangement.In this study we aim to outline and characterized the P. stuartii species. To do so, we retrieved from the GenBank database all genomes labelled as Providencia and performed a comprehensive genome-based species definition founded on average nucleotide identity (ANI) and on alignment-free approaches. After defining the genomes assuredly identifiable as P. stuartii, we devised a MultiLocus Sequence Typing (MLST) and a core-genome MLST (cgMLST) schemes, based on 7 and 2,296 loci respectively.This work hence provides a framework for understanding the role of P. stuartii and of other members of this genus, which should be considered as emerging multidrug-resistant pathogens.

    Keywords: Providencia stuartii, mlst, phylogenomics, gene, allele Repositories: BioProject PRJNA948429 ORCID: Gabriele Arcari 0000-0001-9629-4637 Alessandra Carattoli 0000-0002-6120-6526 Valerio Capitani 0009-0008-4569-1972

    Received: 09 Sep 2024; Accepted: 17 Oct 2024.

    Copyright: © 2024 Arcari, De Francesco, Polani, Carattoli and Capitani. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gabriele Arcari, University of Insubria, Varese, Italy

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.