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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1492127

Enhancing D-Lactic Acid Production from Non-Detoxified Corn Stover Hydrolysate via Innovative F127-IEA Hydrogel-Mediated Immobilization of Lactobacillus bulgaricus T15

Provisionally accepted
Yuhan Zheng Yuhan Zheng 1Feiyang Sun Feiyang Sun 1Siyi Liu Siyi Liu 1Gang Wang Gang Wang 1,2*Huan Chen Huan Chen 1,2Yongxin Guo Yongxin Guo 3,4Xiufeng Wang Xiufeng Wang 5Maia Lia Escobar Bonora Maia Lia Escobar Bonora 1Sitong Zhang Sitong Zhang 1,2Yanli Li Yanli Li 1,2Guang Chen Guang Chen 1,2
  • 1 College of Life Sciences, Jilin Agricultural University, Changchun, Jilin Province, China
  • 2 Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education, Jilin，Changchun, China
  • 3 University of Chinese Academy of Sciences, Beijing, Beijing, China
  • 4 Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences (CAS), Changchun, Hebei Province, China
  • 5 Vegetable and flower Science Research Institute of Jilin Province, 吉林，长春, China

The final, formatted version of the article will be published soon.

    The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable Lactobacillus bulgaricus clusters within the gel matrix. It also maintains excellent mechanical properties. It also maintains excellent mechanical properties. F127-IEA immobilized Lactobacillus bulgaricus T15 (F127-IEA-T15) can be used in cell-recycle fermentation for over 150 days from glucose and 50 days from corn stover hydrolysate, achieving high production rates of D-LA from glucose (2.71 ± 0.85 g/L h) and corn stover hydrolysate (1.29 ± 0.39 g/L h). F127-IEA-T15 enhanced D-LA production by adsorbing and blocking toxic substances present in corn stover hydrolysate that are detrimental to cellular activity.The newly developed hydrogels in this study provide a robust platform for large-scale extraction of D-LA from non-detoxified corn stover.

    Keywords: corn stover, F127-IEA, Phenolic resistance, Immobilization, Cell-recycle fermentation Corn stover, Cell-recycle fermentation

    Received: 06 Sep 2024; Accepted: 11 Nov 2024.

    Copyright: © 2024 Zheng, Sun, Liu, Wang, Chen, Guo, Wang, Escobar Bonora, Zhang, Li and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gang Wang, College of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.