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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiotechnology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1491174
This article is part of the Research Topic Microbial Biotechnologies for Efficient Wastewater Treatment and Resource Recovery View all 8 articles

Efficient low-temperature wastewater treatment by Pseudomonas zhanjiangensis sp. nov.: a novel cold-tolerant bacterium isolated from mangrove sediment

Provisionally accepted
Ming Li Ming Li 1*Xixi Hu Xixi Hu 1*Tiancheng Ni Tiancheng Ni 2*Yuan Ni Yuan Ni 1*Changran Li Changran Li 1*Dong Xue Dong Xue 1*Feng Li Feng Li 1*
  • 1 Anhui University of Chinese Medicine, Hefei, China
  • 2 Nanjing University, Nanjing, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    A novel heterotrophic, cold-tolerant bacterium, designated Pseudomonas zhanjiangensis 25A3E T , was isolated from mangrove sediment and demonstrated excellent efficiency in cold wastewater treatment. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain 25A3E T within the genus Pseudomonas, showing the highest similarity (98.7%) with Pseudomonas kurunegalensis LMG 32023 T . Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were below the species delineation thresholds (70% for dDDH, 95% for ANI), indicating that strain 25A3E T represents a novel species. This strain demonstrated high efficiency in removing nitrogen (N) and organic pollutants under low-temperature conditions. Specifically, it achieved 72.9% removal of chemical oxygen demand (COD), 70.6% removal of ammoniacal nitrogen (NH4 + -N), and 69.1% removal of total nitrogen (TN) after 96 hours at 10°C. Genomic analysis identified key genes associated with cold adaptation, nitrogen removal and organic matter degradation. These findings indicate that Pseudomonas zhanjiangensis 25A3E T holds significant potential for application in cold temperature wastewater treatment, offering a promising solution for environmental remediation in regions with low ambient temperatures.

    Keywords: wastewater treatment, cold tolerant, nitrogen removal, Pseudomonas zhanjiangensis, Comparative genomic analysis

    Received: 04 Sep 2024; Accepted: 17 Oct 2024.

    Copyright: © 2024 Li, Hu, Ni, Ni, Li, Xue and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ming Li, Anhui University of Chinese Medicine, Hefei, China
    Xixi Hu, Anhui University of Chinese Medicine, Hefei, China
    Tiancheng Ni, Nanjing University, Nanjing, 210093, Jiangsu Province, China
    Yuan Ni, Anhui University of Chinese Medicine, Hefei, China
    Changran Li, Anhui University of Chinese Medicine, Hefei, China
    Dong Xue, Anhui University of Chinese Medicine, Hefei, China
    Feng Li, Anhui University of Chinese Medicine, Hefei, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.