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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1490255

Pathological investigation, molecular characterization and first-time isolation of the predominant serotypes of fowl adenovirus (FAdV-D and E) from commercial poultry in Bangladesh

Provisionally accepted
Mohammad Sadekuzzaman Mohammad Sadekuzzaman 1Md Sojon Miah Md Sojon Miah 2Rokshana Parvin Rokshana Parvin 2Dr. Md. Enamul Haque Dr. Md. Enamul Haque 2Sanzila Hossain Sigma Sanzila Hossain Sigma 2Sajedul Hayat Sajedul Hayat 2Muhammad Tofazzal Hossain Muhammad Tofazzal Hossain 2Md. Alimul Islam Md. Alimul Islam 2*
  • 1 Central Disease Investigation Laboratory, Department of Livestock Services, Dhaka, Dhaka, Bangladesh
  • 2 Bangladesh Agricultural University, Mymensingh, Dhaka, Bangladesh

The final, formatted version of the article will be published soon.

    Background: Fowl adenovirus (FAdV) is a globally distributed virus that inflicts significant economic losses on the poultry industry. The study aimed at pathological investigation, molecular characterization, isolation, and pathogenicity determination of FAdV from commercial poultry.Methods: A total of 86 liver samples were collected from 80 commercial chicken farms. All samples were examined for gross pathology, liver, and kidney for histopathology, and the only liver samples were used for virus detection by PCR. PCR-positive FAdV samples were used for isolation of virus in 10-day-old seronegative embryonated chicken eggs (ECEs) via chorioallantoic membrane (CAM). PCR confirmed virus isolates were sequenced for serotyping and phylogenetic analysis. Pathogenicity of the isolated FAdVs was assessed by oral, i.m., and i.p. routes of infection.The study observed gross lesions including hydropericardium and enlarged, friable pale livers with ecchymotic hemorrhages. Histopathological analysis revealed hepatocytic necrosis with basophilic intranuclear inclusion bodies in liver tissues, and tubular necrosis, focal hemorrhages, and mononuclear cell infiltration in kidney tissues. Out of 86 liver samples analyzed, 40 were positive for FAdVs by PCR, and 22 were positive for virus isolation. One serotype was 11 and other eleven were 8b of FAdV and genetically close to Bangladesh, India, and Turkey isolates, according to hexon gene phylogenetic analysis. The pathogenicity test indicated that serotype 11 was more virulent than the serotype 8b.The study concluded that serotypes 11 and 8b of FAdVs are circulating simultaneously among commercial broiler and layer chickens, serotype 8b was found predominant one.

    Keywords: Fowl adenoviruses (FAdV), histopathology, PCR, phylogenetic analysis, Serotypes-8b and 11

    Received: 02 Sep 2024; Accepted: 24 Oct 2024.

    Copyright: © 2024 Sadekuzzaman, Miah, Parvin, Haque, Sigma, Hayat, Hossain and Islam. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Md. Alimul Islam, Bangladesh Agricultural University, Mymensingh, 2202, Dhaka, Bangladesh

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