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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1486566
This article is part of the Research Topic Insights into the Gut Microbiota in Companion Animals View all 6 articles

Gut Microbiome and Metabolome Library Construction based on Age Group Using Short-read and Long-read Sequencing Techniques in Korean Traditional Canine Species Sapsaree

Provisionally accepted
Son Seonhui Son Seonhui 1Min-Geun Kang Min-Geun Kang 1Anna Kang Anna Kang 1Yonggu Kang Yonggu Kang 2Kimoon Kim Kimoon Kim 2Min-Jin Kwak Min-Jin Kwak 3Minho Song Minho Song 2Younghoon Kim Younghoon Kim 1*
  • 1 Seoul National University, Seoul, Republic of Korea
  • 2 Chungnam National University, Daejeon, Daejeon, Republic of Korea
  • 3 University of Florida, Gainesville, Florida, United States

The final, formatted version of the article will be published soon.

    This study investigated age-related changes in the gut microbiota and metabolome of Sapsaree dogs through metagenomic and metabolomic analyses. Using Illumina (short-read) and Nanopore (longread) sequencing technologies, we identified both common and unique bacterial genera in the dogs across different age groups. In metagenomic analysis, Firmicutes were predominant at the family level. At the genus level, Lactobacillus, Streptococcus, Romboutsia, and Clostridium XI were the most abundant, and the bacterial genera typically considered beneficial were less prevalent in senior dogs, whereas the genera associated with pathogenicity were more abundant. These findings suggest age-related shifts in gut microbiota composition. Metabolomic analysis showed distinct clustering of metabolites based on the age group, with changes in metabolite profiles correlating with metagenomic findings. Although Illumina and Nanopore methods provided distinctive results, the genera detected by both methods exhibited similar trends across all age groups in Sapsaree dogs. These findings highlight the relationship between ages, metabolite profiles and gut microbiota composition in dogs, suggesting the need for further research to explore this relation in greater depth.

    Keywords: microbiome, Metabolome, Sapsaree, Illumina sequencing, Nanopore sequencing 1

    Received: 26 Aug 2024; Accepted: 19 Nov 2024.

    Copyright: © 2024 Seonhui, Kang, Kang, Kang, Kim, Kwak, Song and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Younghoon Kim, Seoul National University, Seoul, 151-742, Republic of Korea

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