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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1486501

Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet

Provisionally accepted
Jia-Yu Wang Jia-Yu Wang 1Yaotong Hao Yaotong Hao 1Li-Han Zhang Li-Han Zhang 1*Xiao-Wei Gao Xiao-Wei Gao 1Yi-Huan Xu Yi-Huan Xu 1Jiang-Jiang Wang Jiang-Jiang Wang 1Hanafiah Fazhan Hanafiah Fazhan 2Khor Waiho Khor Waiho 2Yanfeng Sun Yanfeng Sun 1*Cheng-Bin Wu Cheng-Bin Wu 1*
  • 1 Ocean College, Hebei Agricultural University, Qinhuangdao, China
  • 2 Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia

The final, formatted version of the article will be published soon.

    Mandarin fish (Siniperca chuatsi) fed with an artificial diet is progressively gaining popularity, which is important for reducing product prices and resource consumption. However, food is the decisive factor of intestinal microbes, and the profound effects of change in their feeding habit on intestinal microbes of mandarin fish have not been revealed. In the present study, live bait fish and artificial diet were used to feed mandarin fish for 8 weeks to study the effect of different feeding habits on the histology, microbiota structure and dominant bacteria of gut in mandarin fish. The results indicated that feeding with the artificial diet significantly increased the intestinal villi height and muscular thickness in the hindgut of mandarin fish. In addition, the microbiota results showed that there were significant differences of beta diversity of gut microbiota in mandarin fish fed with different diets. At the genus level, feeding artificial diets significantly increased the abundance of Weissella in the mandarin fish gut. Furthermore, two Weissella bacteria were identified and characterized from the midgut contents of mandarin fish fed with artificial diet. Based on 16S rRNA gene sequencing, nine strains were assigned as Weissella cibaria (RM125), and one as Weissella confusa (SJ548).Biochemical analyses based on the VITEK 2 method revealed a pattern of metabolic activities against W.confusa RM125 and W. cibaria SJ548, with 13 positive and 29 negative results, respectively. W. confusa RM125 and W. cibaria SJ548 exhibited sensitivities towards a variety of pathogens, including V. harveyi, S. aureus and V. parahaemolyticus, E. coli, A. hydrophila, S. enterica, V. anguillarum and V. alginolyticus, indicating potential probiotics. Therefore, our results confirmed that the transformation of feeding habit altered the structure, microbial composition and dominant bacteria in gut of mandarin fish, and provided evidence that Weissella might play a crucial role in the digestion and absorption of artificial diet in mandarin fish.

    Keywords: Siniperca chuatsi, artificial diet, Gut histology, Gut Microbiota, Weissella

    Received: 27 Aug 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Wang, Hao, Zhang, Gao, Xu, Wang, Fazhan, Waiho, Sun and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Li-Han Zhang, Ocean College, Hebei Agricultural University, Qinhuangdao, China
    Yanfeng Sun, Ocean College, Hebei Agricultural University, Qinhuangdao, China
    Cheng-Bin Wu, Ocean College, Hebei Agricultural University, Qinhuangdao, China

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