AUTHOR=Takeuchi Kasumi , Ogiso Masayo , Ota Arisa , Nishimura Kentaro , Nishino Chihiro , Omori Yasuhiro , Maeda Mitsunori , Mizui Ryousuke , Yamanaka Homare , Ogino Tomokazu , Seo Shigemi 

TITLE=Pseudomonas rhodesiae HAI-0804 suppresses Pythium damping off and root rot in cucumber by its efficient root colonization promoted by amendment with glutamate

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1485167

DOI=10.3389/fmicb.2024.1485167

ISSN=1664-302X

ABSTRACT=<p>Plant diseases caused by soil-borne fungi and oomycetes significantly reduce yield and quality of many crops in the agricultural systems and are difficult to control. We herein examine <italic>Pseudomonas rhodesiae</italic> HAI-0804, a bacterial biological control agent that was originally developed for control of bacterial diseases on the surface of vegetables, and assessed its efficacy at controlling soil-borne diseases caused by oomycetes. Strain HAI-0804 did not exhibit detectable antibiotic activity toward <italic>Pythium ultimum</italic>, a causal agent of damping-off and root rot; however, it effectively protected against <italic>Pythium</italic> damping-off and root rot in cucumber. Exogenous glutamate enhanced the efficacy of biocontrol, the production of siderophore pyoverdine, root colonization in cucumber plants, and the ratio of biofilm formation to planktonic cells. The epiphytic fitness of strain HAI-0804 appears to contribute to plant protection efficacy against a broad spectrum of pathogens for both above-ground plant parts and the rhizosphere.</p>