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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1483637

Mycosynthesis of silver nanoparticles from endophytic Aspergillus parasiticus and their antibacterial activity against Methicillin resistance Staphylococcus aureus in vitro and in vivo

Provisionally accepted
Enas M. Ali Enas M. Ali Peramaiyan Rajendran Peramaiyan Rajendran Basem M Abdallah Basem M Abdallah *
  • King Faisal University, Al-Ahsa, Eastern Province, Saudi Arabia

The final, formatted version of the article will be published soon.

    Background : Methicillin-resistant Staphylococcus aureus (MRSA) is a drug-resistant and biofilm-forming pathogenic bacteria with severe morbidity and mortality. MRSA showed resistance against current available antibiotics. Thus, there is an urgent need to develop novel effective treatments with minimal side effects to eliminate MRSA.In this study, we aimed to mycosynthesize silver nanoparticles (AgNPs) using endophytic fungus Aspergillus parasiticus isolated from leaves of Reseda Arabica, and to examine their antibacterial activity against MRSA.Results: Screening of fungal secondary metabolites using Gas chromatography-Mass Spectroscopy (GC-MS) analysis revealed the presence of high content of bioactive compounds with antibacterial activities. AP -AgNPs were mycosynthesized for the first time using ethyl acetate extract of A. parasiticus and characterized by imaging (transmission electron microscopy (TEM), UV-VIS spectroscopy, zeta potential, X-ray diffraction (XRD), Energy dispersive x-ray analysis (EDX), and Fourier transform infrared spectroscopy (FTIR). The agar well diffusion method revealed the antibacterial activity of AP-AgNPs against MRSA with MIC 25 µg/ml. AP-AgNPs showed to exert antibacterial action via a bactericidal mechanism based on flow cytometry, scanning electron microscopy, and transmission electron microscopy assessment. Our data demonstrated the effective interaction of AP -AgNPs with the bacterial cell membrane resulted in cell membrane damage and disruption of cell surface structure. Further, AP-AgNP s successfully prevented the development of MRSA biofilms by disturbing cell adhesion and destructing mature biofilm reaching to over 80% clearance rate. Interestingly, topical application of AP-AgNPs to superficial skin infection induced by MRSA in mice, effectively promoted wound healing and suppressed bacterial burden.Our results provide a novel green nanoparticle-drug design with effective therapeutic potential against MRSA-induced skin infection.

    Keywords: silver nanoparticles, MRSA, Aspergillus parasiticus, Skin Infection, Wound Healing

    Received: 20 Aug 2024; Accepted: 05 Nov 2024.

    Copyright: © 2024 Ali, Rajendran and Abdallah. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Basem M Abdallah, King Faisal University, Al-Ahsa, 31982, Eastern Province, Saudi Arabia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.