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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1483633
This article is part of the Research Topic Horizontal Transfer of Antibiotic Resistance Genes in the Environment: Dynamic, Contributing Factors, and Control View all 5 articles

Prevalence and Characterization of IncQ1α-Mediated Multidrug Resistance in Proteus mirabilis Isolated from Pigs in Kunming, China

Provisionally accepted
Hongmei Liu Hongmei Liu 1,2,3Na Xia Na Xia 2*Fanan Suksawat Fanan Suksawat 3*Bundit Tengjaroenkul Bundit Tengjaroenkul 3*Yue Hu Yue Hu 2*Xiaofeng Zhou Xiaofeng Zhou 2*Xiaojiang Li Xiaojiang Li 2*Cuiqin Huang Cuiqin Huang 1*Yinli Bao Yinli Bao 1Qiong Wu Qiong Wu 1*Chunrong Zhang Chunrong Zhang 1*Sunpetch Angkititrakul Sunpetch Angkititrakul 3*Bin Xiang Bin Xiang 2*Xin Wu Xin Wu 2*
  • 1 Engineering Research Center for the Prevention and Control of Animal Original Zoonosis of Fujian Province University, College of Life Science, Longyan University, fujian, China
  • 2 Yunnan Agricultural University, Kunming, Yunnan, China
  • 3 Faculty of Veterinary Medicine, Khon Kaen University, KhonKaen, Thailand

The final, formatted version of the article will be published soon.

    Background: Proteus mirabilis is a conditionally pathogenic bacterium, inherently resistant to polymyxin and tigecycline. Due to the antibiotic resistance genes (ARGs). Theycan horizontally transfer to other bacteria. The possibility of Inc plasmid-mediated ARGs horizontal transmission from Proteus mirabilis poses a serious threat to public health safety. This study aims to know the presence of the Inc plasmid type in the pig-derived Proteus mirabilis in Kunming, Yunnan, China.The fecal samples were collected from pig farms in six districts of Kunming (Luquan, Jinning, Yiliang, Anning, Songming and Xundian) from 2022 to 2023. Isolated Proteus mirabilis was identified by ids and 16S rRNA genes, then positive strains were analyzed by antimicrobial susceptibility test, and incompatibility plasmid typing. The isolates that were multi-drug resistant and the characteristic genes of incompatibility plasmid positive were selected for whole genome sequencing. Then isolates information of resistance and Inc groups was compared with 126 completely level genome public data. The whole genome of the isolate was subjected to whole genome multi-locus sequence typing, resistance group analysis, genome island prediction, and plasmid structural gene analysis.Results: Thirty isolates were obtained from 230 samples, with a prevalence of 13.04%. All isolates were multi-drug resistant, with 100% resistance to cotrimoxazole, erythromycin, penicillin G, chloramphenicol, ampicillin, and streptomycin. Among them, 15 isolates were positive for IncQ1α plasmid repC gene. The top two multi-drug resistance and repC positive strains NO. 15 and 21 were sequenced to compare genomic features on Inc groups and ARGs with the public data. Genome data analysis revealed that all repC is primarily composed of IncQ1α, with structural genes of other F-type plasmids (

    Keywords: TRAV, Trau, tran, TRAL, TRAK, traI, TraH, Trag

    Received: 20 Aug 2024; Accepted: 07 Nov 2024.

    Copyright: © 2024 Liu, Xia, Suksawat, Tengjaroenkul, Hu, Zhou, Li, Huang, Bao, Wu, Zhang, Angkititrakul, Xiang and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Na Xia, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
    Fanan Suksawat, Faculty of Veterinary Medicine, Khon Kaen University, KhonKaen, 40002, Thailand
    Bundit Tengjaroenkul, Faculty of Veterinary Medicine, Khon Kaen University, KhonKaen, 40002, Thailand
    Yue Hu, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
    Xiaofeng Zhou, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
    Xiaojiang Li, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
    Cuiqin Huang, Engineering Research Center for the Prevention and Control of Animal Original Zoonosis of Fujian Province University, College of Life Science, Longyan University, fujian, China
    Qiong Wu, Engineering Research Center for the Prevention and Control of Animal Original Zoonosis of Fujian Province University, College of Life Science, Longyan University, fujian, China
    Chunrong Zhang, Engineering Research Center for the Prevention and Control of Animal Original Zoonosis of Fujian Province University, College of Life Science, Longyan University, fujian, China
    Sunpetch Angkititrakul, Faculty of Veterinary Medicine, Khon Kaen University, KhonKaen, 40002, Thailand
    Bin Xiang, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
    Xin Wu, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

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