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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1483527

IRF7-deficient MDBK cell based on CRISPR/Cas9 technology for enhancing IBRV replication

Provisionally accepted
Yang Gui Ge Yang Gui Ge 1Li Dongli Li Dongli 2Qian Ling Qian Ling 3*Hui Li Xu Hui Li Xu 1*Lin Xiao Wang Lin Xiao Wang 1*Yan Ke Li Yan Ke Li 1*Wen Hao Wen Hao 1*Qing-Long Gong Qing-Long Gong 1Jian-Ming Li Jian-Ming Li 3Kun Shi Kun Shi 3Xue Leng Xue Leng 3*Rui Du Rui Du 3*
  • 1 College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
  • 2 Wengniute banner Agriculture and Animal Husbandry Bureau, Chifeng, China
  • 3 College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China

The final, formatted version of the article will be published soon.

    Infectious bovine rhinotracheitis (IBR), characterized by acute respiratory lesions in cattle, is a major infectious disease caused by bovine alphaherpesvirus-1 (BoAHV-1). Control of this disease is primarily depending on vaccination. Madin-Darby bovine kidney cells (MDBK) being the main host cells and the important production platform for IBR vaccines. However, innate immune genes inhibit viral replication. Accordingly, the aim of this study was developing of IRF7 gene deleted MDBK cells to facilitate the production of high-titer vaccines. The CRISPR/Cas9 technology was used to knock out the IRF7 gene in MDBK cells and the impact on virus replication was examined using virus growth curves, CCK-8 assays, cell scratch assays, and qPCR. The knockout of the IRF7 gene in MDBK cells led to an increased replication capacity of IBRV and a significant reduction in type I interferons expression, specifically IFN-α and IFN-β. This indicates that IRF7 -/-MDBK cell lines can effectively result in production of IBRV with high-titer, which will enhance the development of inactivated or attenuated vaccines.

    Keywords: CRISPR/Cas9, gene knockout, IRF7, IBRV, viral replication

    Received: 20 Aug 2024; Accepted: 05 Nov 2024.

    Copyright: © 2024 Ge, Dongli, Ling, Xu, Wang, Li, Hao, Gong, Li, Shi, Leng and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qian Ling, College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China
    Hui Li Xu, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
    Lin Xiao Wang, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
    Yan Ke Li, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
    Wen Hao, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
    Xue Leng, College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China
    Rui Du, College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China

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