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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1481162

Prevalence and Genetic Diversity of optrA-Positive Enterococci Isolated from Patients in an Anorectal Surgery Ward of a Chinese Hospital

Provisionally accepted
yuanyuan Li yuanyuan Li 1Tao Jiang Tao Jiang 1*Jianfeng Mao Jianfeng Mao 2,3*Fangyi Xu Fangyi Xu 3*Rong Zhang Rong Zhang 4jing Yan jing Yan 3*Jiachang Cai Jiachang Cai 5yanjun xie yanjun xie 3*
  • 1 Department of Clinical Laboratory, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China, Luoyang, China
  • 2 Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Jiangsu Province, China
  • 3 Department of Clinical Laboratory, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China, Hangzhou, China
  • 4 Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang, China, Hangzhou, Jiangsu Province, China
  • 5 Department of Clinical Laboratory, Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu, China, Lanzhou, Gansu Province, China

The final, formatted version of the article will be published soon.

    Linezolid-resistant enterococci has increased in recent years due to worldwide spread of acquired resistance genes (cfr, optrA, and poxtA) in clinical, animal, and environmental settings. This study investigated the carriage of optrA-positive enterococci among patients in the anorectal surgery ward in Hangzhou, China, and characterized the genetic context of optrA. A total of 173 wound secretion samples were obtained to screen optrA-positive enterococci. Of the 173 samples, 15 (8.67%) were positive for optrA, including 12 Enterococcus faecalis, two E. faecium, and one E.hirae. Multilocus sequence type analysis revealed that 12 optrA-positive E. faecalis isolates belonged to eight different sequence types (STs), of which ST16 was the main type. Eight OptrA variants were identified, whose optrA flanking regions with a fexA gene downstream were bounded by different mobile genetic element. Furthermore, the optrA gene in eight out of fifteen optrA-positive enterococci could be successfully transferred through conjugation. The findings revealed a high carriage rate of optrA in enterococci from one anorectal surgery ward in China. The dissemination of optrApositive enterococci isolates in clinical settings should be continually monitored.

    Keywords: optrA, Linezolid resistance, Enterococci, genetic context, Hospital

    Received: 15 Aug 2024; Accepted: 09 Oct 2024.

    Copyright: © 2024 Li, Jiang, Mao, Xu, Zhang, Yan, Cai and xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Tao Jiang, Department of Clinical Laboratory, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan, China, Luoyang, China
    Jianfeng Mao, Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Jiangsu Province, China
    Fangyi Xu, Department of Clinical Laboratory, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China, Hangzhou, China
    jing Yan, Department of Clinical Laboratory, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China, Hangzhou, China
    yanjun xie, Department of Clinical Laboratory, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China, Hangzhou, China

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