Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Aquatic Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1479942

Isolation and characterization of a Bdellovibrio bacteriovorus from fish pond water

Provisionally accepted
Qianming Hong Qianming Hong 1Kai Yuan Kai Yuan 2*Mengen Zhang Mengen Zhang 1*Xinrui Yang Xinrui Yang 1*Qi Chen Qi Chen 1*Shiwei Xu Shiwei Xu 1*Wanyi Chen Wanyi Chen 1*Shixin Qian Shixin Qian 1*Yutao Miao Yutao Miao 1*Zhiming Zhu Zhiming Zhu 3*Yihong Chen Yihong Chen 1*
  • 1 South China Normal University, Guangzhou, China
  • 2 Huizhou University, Huizhou, Guangdong Province, China
  • 3 Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China

The final, formatted version of the article will be published soon.

    The intricate habitats of aquatic organisms, coupled with the prevalence of pathogens, contribute to a high incidence of various diseases, particularly bacterial infections. Consequently, the formulation of sustainable and effective disease management strategies is crucial for the thriving aquaculture sector. In this investigation, a strain of Bdellovibrio bacteriovorus, designated B. bacteriovorus FWA, was isolated from a freshwater fish pond. Identification was achieved through microscopic examination of morphological characteristics, biochemical property assessment, and phylogenetic analysis. The lysogenic capability of B. bacteriovorus FWA was evaluated, revealing its effectiveness in lysing Escherichia coli, Aeromonas hydrophila, Vibrio alginolyticus, Vibrio parahaemolyticus, and Edwardsiella tarda. Physiological analysis indicated that the optimal ratio of B. bacteriovorus FWA to host bacteria was 1:10,000, with strict aerobic requirements. The optimal pH range for growth and reproduction was 7.0 ~ 8.0, the ideal temperature was found to be 30 ~ 35°C, with a preferred Na+ concentration of 0% and a Ca2+ concentration of 15 ~ 25 mM. Additionally, B. bacteriovorus FWA demonstrated enhanced lytic activity against bacteria in aquaculture effluent while effectively managing ammonia-nitrogen levels. In summary, B. bacteriovorus FWA holds significant promise for development as a probiotic agent in aquaculture.

    Keywords: Bdellovibrio bacteriovorus, Aquaculture, Disease, pathogenic bacteria, Aquaculture effluent treatment, lysed properties

    Received: 13 Aug 2024; Accepted: 20 Nov 2024.

    Copyright: © 2024 Hong, Yuan, Zhang, Yang, Chen, Xu, Chen, Qian, Miao, Zhu and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Kai Yuan, Huizhou University, Huizhou, Guangdong Province, China
    Mengen Zhang, South China Normal University, Guangzhou, China
    Xinrui Yang, South China Normal University, Guangzhou, China
    Qi Chen, South China Normal University, Guangzhou, China
    Shiwei Xu, South China Normal University, Guangzhou, China
    Wanyi Chen, South China Normal University, Guangzhou, China
    Shixin Qian, South China Normal University, Guangzhou, China
    Yutao Miao, South China Normal University, Guangzhou, China
    Zhiming Zhu, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China
    Yihong Chen, South China Normal University, Guangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.