Skip to main content

ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1479580
This article is part of the Research Topic Soil Microbial Communities to Promote Suppressiveness against Soil-Borne Pathogens and Diseases View all 6 articles

Understanding the Influence of Plant Genetic Factors on Rhizosphere Microbiome Assembly in Panax notoginseng

Provisionally accepted
Liping Shi Liping Shi 1Mingming Yang Mingming Yang 1Guangfei Wei Guangfei Wei 1Xiuye Wei Xiuye Wei 2Fei Hong Fei Hong 3Jiaxiang Ma Jiaxiang Ma 1Zhe Wu Zhe Wu 1Yuqing Zheng Yuqing Zheng 3Miyi Yang Miyi Yang 1Shilin Chen Shilin Chen 1Guozhuang Zhang Guozhuang Zhang 1*Linlin Dong Linlin Dong 1
  • 1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
  • 2 Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China
  • 3 Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd, Zhangzhou, Fujian Province, China

The final, formatted version of the article will be published soon.

    Functional rhizosphere microbiomes (FRM) are critical for plant health and yield.However, the ecological succession of FRM and their links to plant genetic factors across the life cycle of perennial plants remain poorly understood. This study profiled FRM, including plant-beneficial bacteria (PBB) and fungal plant pathogens (FPP), across different developmental stages of Panax notoginseng. The biodiversity of both PBB and FPP were significantly higher in rhizosphere compared with farmland soil, and exhibited different succession patterns with plant growth. The relative abundance of PBB, but not FPP, decreased after plant cultivation. There were significantly negative correlations between FPP and PBB, particularly the biocontrol subgroup (ρ = -0.56, P < 0.001). The antagonistic effects of biocontrol bacteria against fungal pathogens were further validated by in vitro assays. The fitting of neutral community model indicated that the deterministic assembly of PBB, especially the biocontrol subgroup, was the strongest at the 3 rd -year root growth stage of P. notoginseng. Plant genes involved in protein export, biosynthesis of alkaloids and amino acids were identified as drivers of the deterministic assembly of biocontrol subcommunity by RNA-Seq analysis. Additionally, a total of 13 transcription factors potentially regulating the expression of these biosynthesis genes were identified through coexpression network. In summary, this study unveils the succession patterns of FRM throughout the life cycle of P. notoginseng and the underlying plant genetic mechanisms, providing valuable insights for developing new plant disease management strategies by manipulating microbes.

    Keywords: rhizosphere soil, functional microbiomes, Biocontrol bacteria, fungal plant pathogens, Community Succession, plant genetic network

    Received: 12 Aug 2024; Accepted: 03 Dec 2024.

    Copyright: © 2024 Shi, Yang, Wei, Wei, Hong, Ma, Wu, Zheng, Yang, Chen, Zhang and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Guozhuang Zhang, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.