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METHODS article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1478218

Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device

Provisionally accepted
Shui-Xian Guo Shui-Xian Guo 1Qing Zhang Qing Zhang 1*Nan-Nan Bai Nan-Nan Bai 1*Pei-Yao Yue Pei-Yao Yue 2*Jingping Niu Jingping Niu 3Cong-Cong Yin Cong-Cong Yin 1*Aiqin Yue Aiqin Yue 2Wei-Jun Du Wei-Jun Du 2*Jin-Zhong Zhao Jin-Zhong Zhao 1*
  • 1 Department of Basic Sciences, Shanxi Agricultural University, Taigu, Jinzhong, China
  • 2 College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China
  • 3 College of Life Science, Shanxi Agricultural University, Jinzhong, Shanxi Province, China

The final, formatted version of the article will be published soon.

    The soybean mosaic disease-caused by the soybean mosaic virus (SMV)-significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7. In this study, a simple and rapid magnetic bead-based RNA extraction method was optimized. Furthermore, a reversetranscription loop-mediated isothermal amplification (RT-LAMP) assay that requires no specialized equipment such as PCR Amplifier was proposed, employing a lateral flow device (LFD) for visual interpretation of SMV-SC7. The RT-LAMP-LFD approach facilitated specificity testing of SMV-SC7. Moreover, the limit of detection (LOD) of this method was as low as 10 -5 ng (2.4 copies). The sensitivity of RT-LAMP-LFD was tenfold higher than that of the colorimetric RT-LAMP method. In 194 field samples tested, the RT-LAMP-LFD detection of the SMV-SC7 had accuracy of 98.45% in comparison to RT-qPCR. In conclusion, the assay exhibited high specificity, sensitivity, and rapidity, enabling economical and portable detection of SMV-SC7 and providing technical support to identify SMV-SC7-infected soybeans.

    Keywords: Soybean, Soybean mosaic virus, virulent strain SC7, magnetic beads-based RNA extraction method, LAMP, Lateral flow device

    Received: 09 Aug 2024; Accepted: 09 Dec 2024.

    Copyright: © 2024 Guo, Zhang, Bai, Yue, Niu, Yin, Yue, Du and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qing Zhang, Department of Basic Sciences, Shanxi Agricultural University, Taigu, Jinzhong, 030000, China
    Nan-Nan Bai, Department of Basic Sciences, Shanxi Agricultural University, Taigu, Jinzhong, 030000, China
    Pei-Yao Yue, College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China
    Cong-Cong Yin, Department of Basic Sciences, Shanxi Agricultural University, Taigu, Jinzhong, 030000, China
    Wei-Jun Du, College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China
    Jin-Zhong Zhao, Department of Basic Sciences, Shanxi Agricultural University, Taigu, Jinzhong, 030000, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.