AUTHOR=Liu Yuan , Yin Chen , Wang Jian , Xing Weihai , Huang Yali , Yan Zhiyu , Chen Jiachen , Han Yu , Zhu Weiran , Zhao Yidi , Zhang Kai , Tian Tingting , Guo Xinru , Yuan Lin , Liu Yang 

TITLE=Characterization of the microbial communities and their correlations with volatile flavor compounds and physicochemical factors in Bashang suancai, a traditional Chinese pickle

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1478207

DOI=10.3389/fmicb.2024.1478207

ISSN=1664-302X

ABSTRACT=<p>Bashang suancai is one of the most wellknown traditional fermented vegetables in North China. The study examined the variations in bacterial diversity, physicochemical properties, and volatile flavor compounds (VFCs) of Bashang suancai over a 7-day fermentation period, utilizing Illumina NovaSeq sequencing and headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The leading bacterial phyla were <italic>Firmicutes</italic>, <italic>Proteobacteria</italic>, and <italic>Cyanobacteria</italic>, while the predominant bacterial species included <italic>Vibrio</italic>, <italic>Lactiplantibacillus</italic>, <italic>Cyanobacteriales</italic>, <italic>Weissella</italic>, and <italic>Latilactobacillus</italic>. The bacterial community diversity decreased significantly following 7 days of fermentation. The microbial profiles were markedly affected by pH, reducing sugar content (RSC), and salt content (SC). A total of 187 VFCs were identified from the specimens. Following 5 days of fermentation, the taste compounds achieved equilibrium, with isothiocyanates, alcohols, and esters predominating among the volatile molecules. Spearman correlation analysis revealed that a strong link between <italic>Latilactobacillus</italic>, <italic>Levilactobacillus</italic>, <italic>Lactiplantibacillus</italic>, <italic>Weissella</italic>, and <italic>Vibrio</italic> with the flavor of pickles. This study established a significant foundation for identifying strains that enhance taste development and improve the nutritional and sensory quality of Bashang suancai.</p>