AUTHOR=Kirchner Miranda , Nunez-Garcia Javier , Duggett Nicholas , Gosling Rebecca J. , Anjum Muna F. 

TITLE=Use of transcriptomics and genomics to assess the effect of disinfectant exposure on the survival and resistance of Escherichia coli O157:H7, a human pathogen

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1477683

DOI=10.3389/fmicb.2024.1477683

ISSN=1664-302X

ABSTRACT=<p>Disinfectants are essential for biosecurity, preventing the persistence and spread of zoonotic pathogens on farms and subsequent human infections. In this study, transcriptomics and genomics were utilised to assess the effect of disinfectant exposure on pathogenic <italic>Escherichia coli</italic>. The exposure of <italic>E. coli</italic> O157:H7 to sub-optimal concentrations of commonly used farm disinfectants elicited changes in both the transcriptome and genome. The transcriptomics identified upregulation of &gt;300 genes and downregulation of &gt;100 genes with functions, which included stress response, metabolism, transcription, transportation, membrane-associated and virulence genes. The phage shock protein (<italic>psp</italic>) operon was highly upregulated in response to a quaternary ammonium compound (QAC)-containing disinfectant, which has not previously been associated with a response to chemical stress. Disinfectant-adapted isolates generated by exposure to sub-lethal disinfectants levels demonstrated resistance to several common antibiotics and decreased sensitivity to biocides. Whole genome sequencing of the mutant strains indicated that they had acquired mutations in the genes associated with the upregulation of the multiple antibiotic resistance (MAR) efflux system (<italic>lon</italic> protease and <italic>marR</italic>) and topoisomerase genes (<italic>gyrA</italic> and <italic>gyrB</italic>). The disinfectant-adapted isolates also exhibited increased expression of transcription, respiration and several pH stress response genes localised in the “acid fitness island.” This study demonstrated that sub-optimal disinfectant concentrations allow <italic>E. coli</italic> O157:H7 to adapt and survive disinfection and develop antibiotic resistance. These changes could have implications for disease treatment and elimination on farms. Although <italic>E. coli</italic> O157:H7 and farm disinfectants were the focus of this study, we believe these findings are also applicable to other settings, including hospitals.</p>