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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1477320

Purification and expression of a novel bacteriocin with against Pseudomonas syringae pv. actinidiae (PSA), Bacteriocin JUQZ-1 secreted by Brevibacillus laterosporus Wq-1, Isolated from Rhizosphere Soil of Healthy Kiwifruit

Provisionally accepted
Shuai Yang Shuai Yang *langbo Yi langbo Yi *yi yang yi yang *danni chen danni chen *qingzhong peng qingzhong peng *
  • College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China

The final, formatted version of the article will be published soon.

    Kiwifruit canker caused by Pseudomonas syringae pv. actinidiae (PSA), has brought huge losses to the kiwifruit industry every year. Due to the drug resistance of PSA, biological control is the most potential control method at present, and the development of biocontrol bacterium against PSA would solve the problem of drug resistance generated during the chemical control of PSA to a certain extent.In this work, a Wq-1 strains was isolated from rhizosphere soil of healthy kiwifruit that it demonstrated excellent inhibitory activity against PSA. Based on the morphology characteristics and phylogenetic analysis of 16S rRNA gene sequence, the isolated strain was identified as Brevibacillus laterosporus Wq-1. The bacteriostatic proteins were isolated from cell-free culture filtrate of strain Wq-1 and determined with molecular weight of about 12 kDa by SDS-PAGE. LC-MS/MS detection revealed that there were several peptides in the target band that were consistent with protein 01021 in the genome. The gene of 01021 protein was cloned into the plasmid pPICZa, and the recombinant bacteriocin was successfully expressed through the Pichia pastoris X33 expression system. The recombinant protein 01021 could effectively inhibit the growth of PSA. This protein was first reported to have Deleted: Actinidiae

    Keywords: Kiwifruit canker, Rhizosphere bacterium, Brevibacillus laterosporus, bacteriocin, biological control

    Received: 07 Aug 2024; Accepted: 08 Nov 2024.

    Copyright: © 2024 Yang, Yi, yang, chen and peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Shuai Yang, College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China
    langbo Yi, College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China
    yi yang, College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China
    danni chen, College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China
    qingzhong peng, College of Biological Resources and Environmental Sciences, Jishou University, Jishou, China

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