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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1477074
This article is part of the Research Topic Tobacco Disease and Biological Control View all 6 articles

3' UTR of tobacco vein mottling virus regulates downstream GFP expression and changes in host gene expression

Provisionally accepted
Zhenqi Sun Zhenqi Sun 1Dongyang Liu Dongyang Liu 2Bin Li Bin Li 3Fangfang Yan Fangfang Yan 4Yuhu Wang Yuhu Wang 1Tianqi YANG Tianqi YANG 1Haijuan Wang Haijuan Wang 1Jiaxin Xu Jiaxin Xu 1Hongyou Zhou Hongyou Zhou 1Mingmin Zhao Mingmin Zhao 1*
  • 1 Inner Mongolia Agricultural University, Hohhot, China
  • 2 Liangshan Zhou company of Sichuan province company of Tobacco Corporation in China, Liangshan Zhou, China
  • 3 Sichuan Province Company of Tobacco Corporation in China, Chengdu, Sichuan Province, China
  • 4 Panzhihua City company of Sichuan province company of Tobacco Corporation in China, Panzhihua, China

The final, formatted version of the article will be published soon.

    Tobacco vein mottling virus (TVMV) is a member of the family Potyviridae. The 3' untranslated region (3'UTR) of viral genomic RNA has been reported to significantly impact viral infection. Nevertheless, the role of the TVMV 3'UTR during viral infection remains unknown.Here, a 3'UTR-GFP expression vector was transiently expressed in Nicotiana benthamiana, in which the 3'UTR of TVMV was introduced upstream of the green fluorescent protein (GFP) gene.Transcriptome sequencing was performed to analyze the genes associated with plant resistance.The effect of the TVMV 3'UTR on GFP expression was studied using an Agrobacterium-mediated transient expression assay, revealing that the TVMV 3'UTR significantly inhibited GFP expression. Transcriptome analysis of differentially expressed genes in 3'UTR-GFP in N. benthamiana was performed to elucidate the why the TVMV 3'UTR inhibited GFP expression. Eighty genes related to plant disease resistance were differentially expressed, including 29 upregulated and 51 downregulated genes. Significantly upregulated genes included those encoding the calcium-binding protein CML24, leucine-rich repeat receptor-like tyrosine-protein kinase, and respiratory burst oxidase homolog protein E. The significantly downregulated genes included calcium-binding protein 7, ethylene-responsive transcription factor 10, endoglucanase 5, and receptor-like protein kinase.

    Keywords: Tobacco, tabacco vein molting virus, RNA-Seq, GFP, 3′UTR

    Received: 07 Aug 2024; Accepted: 01 Oct 2024.

    Copyright: © 2024 Sun, Liu, Li, Yan, Wang, YANG, Wang, Xu, Zhou and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Mingmin Zhao, Inner Mongolia Agricultural University, Hohhot, China

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